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Name :
Human CD24/CD24A Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description :
CD24 or CD24A is a mucin-type glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of B cells, granulocytes, epithelial, neuronal, and muscle cells, and on a range of tumor cells. The precursor of CD24 is cleaved to a short 32 amino acid mature peptide that is anchored to the cell surface by GPI. CD24 modulates B-cell activation responses. CD24 promotes antigen-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T cells, which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 may be a genetic modifier for risk and progression of multiple sclerosis. CD24 serve as a receptor for P-selectin. CD24 is involved in molecular adhesion and metastatic tumor spread by facilitating the interaction with platelet and endothelial cells. CD24 is considered as a tumor marker and high expressions can be detected in epithelial ovarian cancer, breast cancer, non-small cell lung cancer, prostate cancer and pancreatic cancer.

Gene Symbol :

NCBI Gene ID :
100133941

Uniprot Entry :
P25063

Construct Details :
The recombinant human CD24-Fc fusion is expressed as a 260 amino acid protein consisting of Ser27 – Ala58 region of CD24 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Source :
Human cells stably expressing human CD24-Fc and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 SETTTGTSSN SSQSTSNSGL APNPTNATTK AAVDKTHTCP PCPAPELLGG PSVFLFPPKP 70 80 90 100 110 120 KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT 130 140 150 160 170 180 VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC 190 200 210 220 230 240 LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV 250 260 MHEALHNHYT QKSLSLSPGK

M.W. :
Calculated molecular mass (kDa): 29; Estimated by SDS-PAGE under reducing condition (kDa): ~45

Calculated PI :
7.02

Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 35785

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :

Molecule Class :
GPI-Anchored Protein

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
CD24; CD24A

Research Area :
Immunology

Pathway/Disease :
B Cell Activation

Species :
Human

CD Antigen :
CD24

References :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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