Name :
Recombinant Rhesus NKG2D Protein (aa 78-216, His Tag)
Biological Activity :
Background :
KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.
Biological Activity :
1. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat:10679-H03H), The EC50 of human ULBP1-Fch (Cat:10679-H03H) is 0.12-0.28 μg/ml.2. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat:10759-H03H), The EC50 of human MICB-Fch (Cat:10759-H03H) is 33.8-79 ng/ml.
Expression Host :
Rhesus
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
NP_001028061.1
NCBI Gene ID :
Synonyms :
Synonyms :
killer cell lectin-like receptor subfamily K, member 1
Amino Acid Sequence :
Molecular Weight :
The recombinant rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the rhesus NKG2D (NP_001028061.1) (Phe78-Val216) was expressed with a polyhistide tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
KLRK1 Protein, Rhesus; NKG2-D Protein, Rhesus; NKG2D Protein, Rhesus NKG2D 背景信息 KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.
References & Citations :
Houchins J, et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 173: 1017-102.Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428):727-9.Zafirova B, et al. (2011) Regulation of immune cell function and differentiation by the NKG2D receptor. Cell Mol Life Sci. 68(21):3519-29.
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