The persistent threat of aflatoxin B1 (AFB1) contamination in edible vegetable oils necessitates the development of efficient, safe, and scalable detoxification technologies. This study evaluates the practical performance of a Cu-BTC metal-organic framework-derived porous carbonaceous material—specifically C-Cu-BTC MOF-600—in removing AFB1 from real peanut and corn oils under industrially relevant conditions. The focus lies on adsorption efficiency, operational feasibility, and preservation of oil quality parameters.
To assess real-world applicability, two commercially available vegetable oils were spiked with AFB1 at three concentration levels: 10, 20, and 40 µg/kg. C-Cu-BTC MOF-600 was tested as an adsorbent at varying dosages and contact times. Results showed that more than 90% of AFB1 was removed within just 30 minutes, demonstrating rapid kinetics crucial for industrial processing. The required adsorbent dosage varied depending on oil type and contamination level: 2.5 mg/g for peanut oil and 1.5 mg/g for corn oil at 10 µg/kg, increasing to 5.5 mg/g and 4.5 mg/g, respectively, at 40 µg/kg. These findings indicate that C-Cu-BTC MOF-600 is effective even at high contamination levels, with optimal performance observed in corn oil, likely due to differences in matrix composition affecting accessibility of adsorption sites.
High-performance liquid chromatography with fluorescence detection (HPLC-FL) confirmed near-complete removal of AFB1, with recovery rates between 86.3% and 105.0% for peanut oil and 91.2% to 106.0% for corn oil, indicating high analytical reliability. The limit of detection (LOD) was 0.33 ng/mL and the limit of quantification (LOQ) was 1 ng/mL, ensuring sensitive monitoring of residual toxin levels.
Critical to food safety and consumer acceptance is the impact of detoxification on oil quality. Comprehensive analysis revealed no significant changes in key indicators after treatment.IL2RA Antibody manufacturer Acid value (AV), which reflects free fatty acid content, remained stable across all samples.BDH1 Antibody Biological Activity Peroxide value (POV), a marker of lipid oxidation, showed no detectable increase, suggesting no adverse oxidative degradation occurred during the process. Fatty acid profiles—including major components like oleic (C18:1), linoleic (C18:2), palmitic (C16:0), and stearic (C18:0)—were preserved with minimal variation. Vitamin E (VE), a vital antioxidant, retained its original concentration, confirming the adsorbent’s selectivity for AFB1 over essential nutrients. Resveratrol, a bioactive compound present in peanuts, showed a slight decrease, indicating minor co-adsorption, but this effect was limited and did not compromise nutritional value significantly.PMID:34918410
Metallic copper residues were undetectable in treated oils using inductively coupled plasma mass spectrometry (ICP-MS), confirming the safety of the sorbent. Furthermore, cytotoxicity assays using human gastric epithelial GES-1 and NCI-N87 cells demonstrated that both the treated oils and the adsorbent itself exhibited negligible toxicity. Cell viability remained above 80% even at high concentrations of treated oil (up to 50%) and up to 20 µg/mL of C-Cu-BTC MOF-600, indicating excellent biocompatibility.
These results collectively demonstrate that C-Cu-BTC MOF-600 is not only highly effective in AFB1 removal but also maintains the sensory, nutritional, and safety profile of vegetable oils. Its fast action, low required dosage, and compatibility with existing oil processing systems make it a promising candidate for commercial adoption. Future research should explore surface functionalization to further enhance selectivity and reduce non-specific adsorption of beneficial compounds. Overall, this work provides strong evidence for the practical implementation of MOF-derived porous carbons in the food industry for mycotoxin control.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
