Name :
Human B7-H2 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
B7H2 (B7 homolog 2), also known as CD275, B7RP1 (B7related protein) and ICOSL (ICOS Ligand), is a single-pass type I transmembrane glycoprotein that belongs to the B7 family of the immunoglobulin (Ig) superfamily. Like other B7 family members, B7-H25 contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. B7-H2 has been identified as the ligand for ICOS (also known as CD278), a member of the CD28/CTLA4 family of co-stimulatory receptors. The receptor binding of B7-H2 is mediated by the IgV domain and requires the IgC domain for maintaining the structural integrity of the protein. The B7-H2/ICOS interaction plays roles in T cell dependent B cell activation and Th (T helper) cell differentiation, leading to both positive and negative effects on immune responses. In human, B7-H2 also binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate T cells. B7-H2 contributes to the development of allergic asthma and other autoimmune conditions. A soluble form of human B7H2 is elevated in the circulation of patients with systemic lupus erythematosus. In the thyroid, B7H2 is upregulated on thyrocytes during inflammation and promotes the production of thryoid hormones. It is reported that mouse and human B7-H2 show crossspecies binding to ICOS.
Gene Symbol :
The recombinant human B7-H2/ICOSL/CD275-Fc fusion protein is expressed as a 466 amino acid protein consisting of Asp19 – Thr256 region of B7-H2 (UniProt accession #O75144) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.
NCBI Gene ID :
23308
Uniprot Entry :
O75144
Construct Details :
The recombinant human B7-H2/ICOSL/CD275-Fc fusion protein is expressed as a 466 amino acid protein consisting of Asp19 – Thr256 region of B7-H2 (UniProt accession #O75144) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.
Source :
Human cells stably expressing B7-H2-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGD FSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWI NKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVST GEKNAATSTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSL SPGK
M.W. :
Calculated molecular mass 52 kDa; estimated by SDS-PAGE under reducing condition ~75 kDa probably due to glycosylation
Calculated PI :
5.63
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human ICOS and stimulates human T cell proliferation in the presence of anti-CD3.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD275; ICOSL; B7H2; GL50; ICOSLG; B7RP1; ICOS-L; LICOS; B7RP-1; KIAA0653
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD275
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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