Name :
Human CD89/FCAR/FcalphaRI Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
FCAR (Fc alpha receptor) or CD89 is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily (IgSF). ICD89 contains two Ig-like C2-type domains in the extracellular region. CD89 binds to the Fc region of immunoglobulins alpha (IgA) and mediates several functions including cytokine production. CD89 associates with ITAMcontaining signaling subunit FcRγ (Ig epsilon receptor subunit gamma) to form a functional signaling complex. CD89 is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. CD89 recognizes IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity (ADCC), and stimulation of the release of inflammatory mediators. There are multiple alternatively splicing variants encoding different isoforms of CD89.
Gene Symbol :
NCBI Gene ID :
2204
Uniprot Entry :
P24071
Construct Details :
The recombinant human FCAR-Fc protein is expressed as a 433 amino acid protein consisting of Gln22 – Gln226 region of FCAR/CD89 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human FCAR/CD89-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QEGDFPMPFI SAKSSPVIPL DGSVKIQCQA IREAYLTQLM IIKNSTYREI GRRLKFWNET 70 80 90 100 110 120 DPEFVIDHMD ANKAGRYQCQ YRIGHYRFRY SDTLELVVTG LYGKPFLSAD RGLVLMPGEN 130 140 150 160 170 180 ISLTCSSAHI PFDRFSLAKE GELSLPQHQS GEHPANFSLG PVDLNVSGIY RCYGWYNRSP 190 200 210 220 230 240 YLWSFPSNAL ELVVTDSIHQ DYTTQSTGTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS 250 260 270 280 290 300 RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL 310 320 330 340 350 360 NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP 370 380 390 400 410 420 SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN 430 HYTQKSLSLS PGK
M.W. :
Calculated molecular mass (kDa): 48.9; Estimated by SDS-PAGE under reducing condition (kDa): 60-65
Calculated PI :
6.53
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 70415
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
FCAR; CD89; FcalphaRI; CTB-61M7.2
Research Area :
Immunology
Pathway/Disease :
Immune Responses
Species :
Human
CD Antigen :
CD89
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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