Name :
Mouse MSLN/Mesothelin Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
Mesothelin (MSLN) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum. It is overexpressed in mesotheliomas, ovarian and pancreatic cancers as well as in specific squamous cell carcinomas. The MSLN gene encodes a precursor protein that is cleaved into two products, megakaryocyte-potentiating factor (MPF) and MSLN. MPF is secreted and functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. The cleaved form of MSLN remains GPI-anchored on cell-surface and may function as a cell adhesion molecule. Alternative splicing results in multiple transcript variants. Two variant forms exist; variant 1 has an eight amino acid insertion and is rarely expressed, while variant 2 is a truncated form found in cancers but rarely detected in normal individuals. MSLN interacts with CA125/MUC16 and potentiates megakaryocyte colony formation in vitro. MSLN is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.
Gene Symbol :
10 20 30 40 50 60
NCBI Gene ID :
56047
Uniprot Entry :
Q61468
Construct Details :
The recombinant mouse MSLN-Fc fusion protein is expressed as a 536-amino acid protein consisting of Arg296 – Asp592 region of murine MSLN and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Mammalian cells stably expressing mouse MSLN-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 RRDAEQKACP PGKEPYKVDE DLIFYQNWEL EACVDGTMLA RQMDLVNEIP FTYEQLSIFK 70 80 90 100 110 120 HKLDKTYPQG YPESLIQQLG HFFRYVSPED IHQWNVTSPD TVKTLLKVSK GQKMNAQAIA 130 140 150 160 170 180 LVACYLRGGG QLDEDMVKAL GDIPLSYLCD FSPQDLHSVP SSVMWLVGPQ DLDKCSQRHL 190 200 210 220 230 240 GLLYQKACSA FQNVSGLEYF EKIKTFLGGA SVKDLRALSQ HNVSMDIATF KRLQVDSLVG 250 260 270 280 290 300 LSVAEVQKLL GPNIVDLKTE EDKSPVRDWL FRQHQKDLDR LGLGLQGGIP NGYLVLDGST 310 320 330 340 350 360 TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP 370 380 390 400 410 420 EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP 430 440 450 460 470 480 IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY 490 500 510 520 530 KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass (kDa): 60.3; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
6.00
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 76040
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S” and “M” for marker)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
GPI-anchored Protein
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
MSLN; MPF; SMRP; CAK1
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Mouse
CD Antigen :
References :
1. J. Biol. Chem. 269:805-808 (1994) 2. J. Biol. Chem. 270:21984-21990 (1995) 3. Clin. Cancer Res. 12:4225-4231 (2006) 4. Proc. Natl. Acad. Sci. 96:11531-11536 (1999)
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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