Name :
Human PDGFRA Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
PDGFRA (plateletderived growth factor receptor alpha), also known as CD140a and PDGFR2, is a single-pass type I transmembrane glycoprotein in the CSF-1/PDGF receptor subfamily of the receptor tyrosine kinases (RTK) family. Like other family members, PDGFRA contains 5 immunoglobulinlike (Ig) domains in the extracellular region and a split tyrosine kinase domain in the intracellular region. PDGFRA and its closely related homolog PDGFRB can form homo or heterodimeric receptors when engaged by disulfidelinked dimers of the PDGF family of growth factors, including PDGF-A, B, C or D, or the heterodimer PDGFAB that is mainly found in human platelets. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Abnormal PDGFRA signaling in humans may result in spina bifida or cleft palate-type malformations. PDGFRA may also be a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in kidney development. PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment. PDGFRA has been implicated in development and metastasis of liver cancer. PDGFRA is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma). PDGFRA gene amplification may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.
Gene Symbol :
NCBI Gene ID :
5156
Uniprot Entry :
P16234
Construct Details :
The recombinant human PDGFRA-Fc fusion is expressed as a 733 amino acid protein consisting of Gln24 – Ala528 region of PDGFRA and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing PDGFRA-Fc and growing in chemical-defined media with no animal components or antibiotics.
Amino Acid Sequence: :
QLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFV TVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVE DDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQT IPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGIT MLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIK PTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRS KLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCT AEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHPRDRSTVEGRVTFAKVEE TIAVRCLAKNLLGAENRELKLVAPTLRSELTVASTGTHTCPPCPAPELLGGPSVFLFPP KPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNV FSCSVMHEALHNHYTQKSLSLSPGK M.W.: Calculated molecular mass (kDa): 82.1; Estimated by SDS-PAGE under reducing condition (kDa): 90-100 Calculated PI: 5.24 Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 90730 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free). Endotoxin Level: Biological Activity: Binds human PDGF family members (PDGFA, PDGFB and PDGFC) and blocks PDGF-mediated signaling activity in fibroblast cells.
M.W. :
Calculated molecular mass (kDa): 82.1; Estimated by SDS-PAGE under reducing condition (kDa): 90-100
Calculated PI :
5.24
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human PDGF family members (PDGFA, PDGFB and PDGFC) and blocks PDGF-mediated signaling activity in fibroblast cells.
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD140A; PDGF-R-alpha; PDGFR2; PDGFR-2; RHEPDGFRA
Research Area :
Cancer
Pathway/Disease :
PDGF Signaling Pathway
Species :
Human
CD Antigen :
CD140a
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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