Name :
Human NRP2/NP2/NPN2/VEGF165R2/neuropilin2 Protein, ECD (Extracellular Domain), Recombinant
Description :
NRP2 (neuropilin-2), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein. Both members of neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB), 2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members. The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP2 is implicated in cardiovascular development, axon guidance, and tumorigenesis. NPR2 is a high affinity receptor for SEMA3C, SEMA3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
Gene Symbol :
Pro864 region of NRP2 and a C-terminal His-tag. NRP2 ECD contains 4 potential N-linked glycosylation sites.
NCBI Gene ID :
8828
Uniprot Entry :
O60462
Construct Details :
The recombinant human NRP2 ECD protein is expressed as an 855-amino acid protein consisting of Arg21 – Pro864 region of NRP2 and a C-terminal His-tag. NRP2 ECD contains 4 potential N-linked glycosylation sites.
Source :
Human cells stably expressing NRP2 ECD and growing in chemical-defined media with no animal component or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 RGQPDPPCGG RLNSKDAGYI TSPGYPQDYP SHQNCEWIVY APEPNQKIVL NFNPHFEIEK 70 80 90 100 110 120 HDCKYDFIEI RDGDSESADL LGKHCGNIAP PTIISSGSML YIKFTSDYAR QGAGFSLRYE 130 140 150 160 170 180 IFKTGSEDCS KNFTSPNGTI ESPGFPEKYP HNLDCTFTIL AKPKMEIILQ FLIFDLEHDP 190 200 210 220 230 240 LQVGEGDCKY DWLDIWDGIP HVGPLIGKYC GTKTPSELRS STGILSLTFH TDMAVAKDGF 250 260 270 280 290 300 SARYYLVHQE PLENFQCNVP LGMESGRIAN EQISASSTYS DGRWTPQQSR LHGDDNGWTP 310 320 330 340 350 360 NLDSNKEYLQ VDLRFLTMLT AIATQGAISR ETQNGYYVKS YKLEVSTNGE DWMVYRHGKN 370 380 390 400 410 420 HKVFQANNDA TEVVLNKLHA PLLTRFVRIR PQTWHSGIAL RLELFGCRVT DAPCSNMLGM 430 440 450 460 470 480 LSGLIADSQI SASSTQEYLW SPSAARLVSS RSGWFPRIPQ AQPGEEWLQV DLGTPKTVKG 490 500 510 520 530 540 VIIQGARGGD SITAVEARAF VRKFKVSYSL NGKDWEYIQD PRTQQPKLFE GNMHYDTPDI 550 560 570 580 590 600 RRFDPIPAQY VRVYPERWSP AGIGMRLEVL GCDWTDSKPT VETLGPTVKS EETTTPYPTE 610 620 630 640 650 660 EEATECGENC SFEDDKDLQL PSGFNCNFDF LEEPCGWMYD HAKWLRTTWA SSSSPNDRTF 670 680 690 700 710 720 PDDRNFLRLQ SDSQREGQYA RLISPPVHLP RSPVCMEFQY QATGGRGVAL QVVREASQES 730 740 750 760 770 780 KLLWVIREDQ GGEWKHGRII LPSYDMEYQI VFEGVIGKGR SGEIAIDDIR ISTDVPLENC 790 800 810 820 830 840 MEPISAFAGE NFKVDIPEIH EREGYEDEID DEYEVDWSNS SSATSGSGAP STDKEKSWLY 850 TLDPSTGHHH HHHHH
M.W. :
Calculated molecular mass (kDa): 96.4; Estimated by SDS-PAGE under reducing condition (kDa): 100-110
Calculated PI :
5.10
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 161785
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free)
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
NRP2; NP2; NPN2; PRO2714; VEGF165R2; neuropilin-2; neuropilin2
Research Area :
Cancer
Pathway/Disease :
Angiogenesis
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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