Name :
Human ACVRL1/ALK1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. The type I receptors are also referred to as ALKs (Activin receptor-like kinases). ACVRL1 (Activin A receptor type II-like 1), also known as ALK1, HHT2, SKR3, TSR-I, and ACVRLK1, is an endothelial-specific type I receptor in the TGFβ receptor (TGFBR) family. ACVRL1 is expressed highly in endothelial cells and other highly vascularized tissues. ACVRL1 acts as a type I receptor for TGFβ1, TGFβ3, BMP9 and BMP10 and an important regulator of normal blood vessel development or repair. It may bind Activin As well. The expression pattern of ALK1 parallels that of endoglin. Mutations in ACVRL1 as well as in endoglin are associated with hemorrhagic telangiectasia 2(HHT2).
Gene Symbol :
ACVRL1; ALK1; ALK-1; HHT; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
NCBI Gene ID :
94
Uniprot Entry :
P37023
Construct Details :
The recombinant humanACVRL1-Fc fusion protein is expressed as a 325 amino acid protein consisting of Asp22 – Gln118 region of ACVRL1 (UniProt accession #P37023) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as “DTT: -“).
Source :
Human cells stably expressing human ACVRL1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
DPVKPSRGPLVTCTCESPHCKGPTCRGAWCTVVLVREEGRHPQEHRGCG NLHRELCRGRPTEFVNHYCCDSHLCNHNVSLVLEATQPPSEQPGTDGQS TGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHE DPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKE YKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTC LVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 36.3; Estimated by SDS-PAGE under reducing condition (kDa): ~50
Calculated PI :
6.97
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 43400
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized ACVRL1/ALK1 protein binds human BMP9 in a functional ELISA. Inhibits BMP9-induced alkaline phosphatase production by chondrogenic cells.
Molecule Class :
Serine/Threonine Kinase Receptor
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
ALK1; ALK-1; HHT; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
Research Area :
Development
Pathway/Disease :
Activin/Nodal Signaling Pathway
Species :
Human
CD Antigen :
References :
1. Oncogene 8:2879 (1993). 2. Science 264:101 (1994). 3. J. Biol. Chem. 274:9984 (1999). 4. Biochemistry 51:6328 (2012). 5. J. Biol. Chem. 287:27313 (2012). 6. Nat. Genet. 13:189 (1996).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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