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Name :
Human GPC3 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description :
GPC3 (Glypican-3) is a member of the Glypican (GPC) family. Glypicans are a family of heparan sulfate proteoglycans that are linked to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been found in mammals. All of them contain an N­terminal signal peptide, a large globular cysteine­rich domain (CRD) with 14 conserved cysteine residues, a stalk­like region containing the heparan sulfate attachment sites, and a C­terminal GPI anchoring site. GPC3 is processed by convertases that generates at least 3 ploypeptides: the 1st starts with Gln25, the 2nd starts with Ser359 after a furin cleavage site, and the 3rd starts with Val483. Therefore 2-3 different MW species are generated in the recombinant GPC3 preparation. They appear to be linked by disulfide bonds. GPC3 is involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. GPC3 may regulate growth and tumor predisposition. Mutations in GPC3 cause a rare disorder Simpson­Golabi­Behmel Syndrome, which is characterized by pre- and post-natal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors. GPC3 is overexpressed in various cancers including hepatocellular carcinoma and melanoma. GPC3 is implicated in regulating many signaling pathways such as IGF, FGF, BMP and Wnt. GPC3 directly binds FGF­basic by the heparan sulfate chains. GPC3 has been considered as a tumor marker and a potential target for immunotherapy.

Gene Symbol :
20 30 40 50 60

NCBI Gene ID :

Uniprot Entry :

Construct Details :
The recombinant human GPC3 extracellular or ecto-domain (ECD) is expressed as a 762 amino acid protein consisting of Gln25-Val558 region of GPC3 (Uniprot accession# P51654-3) and a C-terminal Fc fusion from human IgG1, which exists as multiple proteolytically processed species under reducing conditions (see the gel image inserted).

Source :
Human cells stably expressing GPC3-Fc growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 QPPPPPPDAT CHQVRSFFQR LQPGLKWVPE TPVPGSDLQV CLPKGPTCCS RKMEEKYQLT 70 80 90 100 110 120 ARLNMEQLLQ SASMELKFLI IQNAAVFQEA FEIVVRHAKN YTNAMFKNNY PSLTPQAFEF 130 140 150 160 170 180 VGEFFTDVSL YILGSDINVD DMVNELFDSL FPVIYTQLMN PGLPDSALDI NECLRGARRD 190 200 210 220 230 240 LKVFGNFPKL IMTQVSKSLQ VTRIFLQALN LGIEVINTTD HLKFSKDCGR MLTRMWYCSY 250 260 270 280 290 300 CQGLMMVKPC GGYCNVVMQG CMAGVVEIDK YWREYILSLE ELVNGMYRIY DMENVLLGLF 310 320 330 340 350 360 STIHDSIQYV QKNAGKLTTT IGKLCAHSQQ RQYRSAYYPE DLFIDKKVLK VAHVEHEETL 370 380 390 400 410 420 SSRRRELIQK LKSFISFYSA LPGYICSHSP VAENDTLCWN GQELVERYSQ KAARNGMKNQ 430 440 450 460 470 480 FNLHELKMKG PEPVVSQIID KLKHINQLLR TMSMPKGRVL DKNLDEEGFE SGDCGDDEDE 490 500 510 520 530 540 CIGGSGDGMI KVKNQLRFLA ELAYDLDVDD APGNSQQATP KDNEISTFHN LGNVSTGTHT 550 560 570 580 590 600 CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH 610 620 630 640 650 660 NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE 670 680 690 700 710 720 PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF 730 740 750 760 LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK (Note: potential proteolytic processing Arg sites are indicated in red) M.W.: Calculated molecular mass (kDa): 86.2; Estimated by SDS-PAGE under reducing condition (kDa): 3 major species at 40, 60 and 95-125 kDa. Calculated PI: 6.11 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 88585 Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Binds human FGF-basic/FGF2 in a functional ELISA. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Nat. Genet. 12: 241 (1996) 2. J. Biol. Chem. 272:7574 (1997) 3. J. Clinical Invest. 108:497 (2001) 4. J. Cell. Biol. 163: 625 (2003) 5. Clin Cancer Res. 10: 6612 (2004) Documentation Product datasheet (pdf) can be downloaded here: FCL2046-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.

M.W. :
: 6.11

Calculated PI :

Calculated Extinction Coefficients :

Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds human FGF-basic/FGF2 in a functional ELISA.

Molecule Class :
GPI-anchored Protein

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :

Research Area :

Pathway/Disease :
IGF Signaling Pathway

Species :

CD Antigen :

References :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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