Name :
Human CD146/MUC18/MCAM Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CD146, also known as MUC18 and MCAM, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. CD146 contains 2 Ig-like V-type domains and 3 Ig-like C2-type domains (V-V-C2-C2-C2) in the extracellular region (ECD) in addition to a transmembrane (TM) region and a short cytoplasmic tail. CD146 is expressed in endothelial cells in vascular tissue throughout the body. Its expression appears to be limited to vascular smooth muscle in normal adult tissues. CD146 is involved in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 is also an adhesion molecule active in neural crest cells during embryonic development. CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 can act as a cell surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1 as well as a transient increase in the intracellular calcium concentration. CD146 is expressed most strongly on metastatic lesions and advanced primary tumors while it is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. It thus suggests that CD146 may play an important role in tumor progression and metastasis.
Gene Symbol :
NCBI Gene ID :
4162
Uniprot Entry :
P43121
Construct Details :
The recombinant human CD146-Fc fusion is expressed as a 774 amino acid protein consisting of Val24 – Gly559 region of CD146 (Uniprot Accession #P43121) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD146-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS 70 80 90 100 110 120 EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV 130 140 150 160 170 180 NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT 190 200 210 220 230 240 LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK 250 260 270 280 290 300 EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL 310 320 330 340 350 360 DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG 370 380 390 400 410 420 QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK 430 440 450 460 470 480 ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN 490 500 510 520 530 540 DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGSTTE 550 560 570 580 590 600 NLYFQGSTGT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV 610 620 630 640 650 660 KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE 670 680 690 700 710 720 KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT 730 740 750 760 770 TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK M.W.: Calculated molecular mass (kDa): 86.4; Estimated by SDS-PAGE under reducing condition (kDa): 90-100 Calculated PI: 5.50 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 94280 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure. Endotoxin Level: Biological Activity: Immobilized CD146-Fc protein supports the adhesion of HUVEC (human umbilical vein endothelial cell) FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Proc. Natl. Acad. Sci. 86:9891 (1988) 2. Melanoma Res. 3:337 (1992) 3. J. Biol. Chem. 276:1564 (2000) 4. Cancer Res. 54:2514 (1993) 5. J. Immunol. 179: 6673 (2007) Documentation Product datasheet (pdf) can be downloaded here: FCL2193B-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 5.50
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized CD146-Fc protein supports the adhesion of HUVEC (human umbilical vein endothelial cell)
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD146; MUC18; MCAM; P1H12; A32
Research Area :
Immunology
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD146
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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