Name :
Human CD226/DNAM1 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
The leukocyte adhesion molecule CD226, also known as DNAM1 (DNAX accessory molecule1) and PTA1 (platelet and T cell activation antigen 1), is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (IgSF). CD226 contains a 236 amino acid (aa) extracellular domain (ECD) with 2 Iglike C2set (IgC2) domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. CD226 is identified as an intercellular adhesion molecule, which is constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. CD226 is expressed on multiple lymphoid and myeloid cells and interacts with the poliovirus receptor CD155/PVR and its family member CD112/Nectin2. Ligation of CD226 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; but inhibits the forrmation of osteoclasts. CD226 is critical for NK cell- and CD8+ T cell-mediated cytotoxicity. It also plays an important role in immune surveillance of tumor development and is involved in triggering naive T cell differentiation and proliferation. Plateletendothelium interactions mediated by CD226 may contribute to the metastasis of tumor cells to the lung.
Gene Symbol :
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
NCBI Gene ID :
10666
Uniprot Entry :
Q15762
Construct Details :
The recombinant human CD226-Fc fusion is expressed as a 466 amino acid protein consisting of Glu19 – Gly256 region of CD226 (Uniprot Accession #Q15762) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD226-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EEVLWHTSVP FAENMSLECV YPSMGILTQV EWFKIGTQQD SIAIFSPTHG MVIRKPYAER 70 80 90 100 110 120 VYFLNSTMAS NNMTLFFRNA SEDDVGYYSC SLYTYPQGTW QKVIQVVQSD SFEAAVPSNS 130 140 150 160 170 180 HIVSEPGKNV TLTCQPQMTW PVQAVRWEKI QPRQIDLLTY CNLVHGRNFT SKFPRQIVSN 190 200 210 220 230 240 CSHGRWSVIV IPDVTVSDSG LYRCYLQASA GENETFVMRL TVAEGKTDNQ YTLFVAGGST 250 260 270 280 290 300 GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG 310 320 330 340 350 360 VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG 370 380 390 400 410 420 QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD 430 440 450 460 GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK M.W.: Calculated molecular mass (kDa): 52.5; Estimated by SDS-PAGE under reducing condition (kDa): 70-75 Calculated PI: 6.34 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 85550 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure. Endotoxin Level: Biological Activity: Immobilized CD226 interacts with Necl5/PVR/CD155 (SKU#FCL1077) and CD112 in a functional ELISA. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Immunity 4:573 (1996) 2. J. Immunol. 166:1671 (1998) 3. J. Exp. Med. 198:557 (2003) 4. J. Biol. Chem. 278:36748 (2003) 5. J. Immunol. 175:1558 (2005) 6. Blood 107:1491 (2006) Documentation Product datasheet (pdf) can be downloaded here: FCL2323B-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.34
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized CD226 interacts with Necl5/PVR/CD155 (SKU#FCL1077) and CD112 in a functional ELISA.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD226
References :
1. Immunity 4:573 (1996) 2. J. Immunol. 166:1671 (1998) 3. J. Exp. Med. 198:557 (2003) 4. J. Biol. Chem. 278:36748 (2003) 5. J. Immunol. 175:1558 (2005) 6. Blood 107:1491 (2006)
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