Name :
Human SCF/KITL/KITLG/KL/MGF/SLF Protein, ECD (Extracellular Domain), Recombinant
Description :
SCF (stem cell factor), also known as KITL/KITLG/KL (ckit ligand), MGF (mast cell growth factor), and steel factor (SLF), is a single-pass type I transmembrane protein belonging to to the SCF family of dimeric transmembrane growth factors. Mature human SCF is composed of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. Proteolytic cleavage releases a 25 kDa soluble protein that shows both Nlinked and Olinked glycosylation. SCF is a primary growth factor that promotes the survival, differentiation, and mobilization of multiple cell types, including mast cells, myeloid, erythroid, megakaryocytic, lymphoid, germ cell, eosinophils and melanocytes. For example, mast cell development depends on SCF and its receptor, c-Kit, which is a receptor tyrosine kinase. Mast cells participate in a variety of immune responses, such as parasite resistance and allergic reactions. SCF also increases the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. The noncovalent dimers of transmembrane or soluble form of SCF interact with ckit to trigger receptor dimerization and signaling activation. SCF may assist in the recovery of cardiac function following myocardial infarction. It also plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin with the potential for CSF-dependent treatment of pathological skin conditions. The mutations in SCF associate wih hyperpigmentation with or without hypopigmentation, familial progressive (FPHH).
Gene Symbol :
NCBI Gene ID :
4254
Uniprot Entry :
P21583
Construct Details :
The recombinant human SCF ECD is expressed as a 179 amino acid protein consisting of Glu26 – Gly184 region of SCF220 isoform (UniProt#P21583-2) and a C-terminal poly-His tag, which exists as a monomer under reducing and non-reducing conditions.
Source :
Human cells stably expressing SCF ECD growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL 70 80 90 100 110 120 DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN 130 140 150 160 170 RSIDAFKDFV VASETSDCVV SSTLSPEKGK AKNPPGDSST TENLYFQGST GHHHHHHHH M.W.: Calculated molecular mass (kDa): 20.2; Estimated by SDS-PAGE under reducing condition (kDa): 30-35 Calculated PI: 5.97 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 11710 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Binds to human c-Kit in a functional ELISA and stimulates TF-1 human erythroleukemic cell proliferation. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Cell 63:203 (1990) 2. J. Biol. Chem. 266:18942 (1991) 3. Proc. Natl. Acad. Sci. 88:4671 (1991) 4. Blood. 99: 1866 (2002) 5. Pigment Cell Res.16: 287 (2003) 6. Cardiovasc. Res. 70:117 (2006) Documentation Product datasheet (pdf) can be downloaded here: FCL2346-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 5.97
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to human c-Kit in a functional ELISA and stimulates TF-1 human erythroleukemic cell proliferation.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
SCF; KITLG; SF; SLF; MGF; FPH2; FPHH; KL-1; Kitl; SHEP7
Research Area :
Stem Cell
Pathway/Disease :
KIT signaling
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CDCP1 Protein
IL-19 Protein
Popular categories:
ADAM15
IGFBP-3
