Name :
Human TPBG/5T4/WAIF1/M6P1/5T4AG Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
TPBG (trophoblast glycoprotein), also known as oncofetal antigen 5T4 and WAIF1 (wnt-activated inhibitory factor 1), is a single-pass type I transmembrane glycoprotein of the LRR (leucine-rich repeat) protein family. TPBG is an oncofetal antigen that is specific to trophoblast cells except for amniotic epithelium. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development. In adults its expression is limited to a few epithelial cell types but highly abundant in many types of tumor cells. TPBG’s expression is associated with poor clinical outcome in numerous cancers. TPBG contains 1 LRRNT (LRR N-terminal cap), 7 LRR (LRR1-7), and 1 LRRCT (LRR C-terminal cap) domains in the extracellular region. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. The C-terminal part of LRRNT and LRR1 are essential for the inhibition of the Wnt signaling pathway. TPBG may also be involved in cell adhesion and motility.
Gene Symbol :
NCBI Gene ID :
7162
Uniprot Entry :
Q13641
Construct Details :
The recombinant human TPBG-Fc fusion is expressed as a 561 amino acid protein consisting of Ser32 – Ser355 region of TPBG and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing condition.
Source :
Human cells stably expressing human TPBG-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 SSPTSSASSF SSSAPFLASA VSAQPPLPDQ CPALCECSEA ARTVKCVNRN LTEVPTDLPA 70 80 90 100 110 120 YVRNLFLTGN QLAVLPAGAF ARRPPLAELA ALNLSGSRLD EVRAGAFEHL PSLRQLDLSH 130 140 150 160 170 180 NPLADLSPFA FSGSNASVSA PSPLVELILN HIVPPEDERQ NRSFEGMVVA ALLAGRALQG 190 200 210 220 230 240 LRRLELASNH FLYLPRDVLA QLPSLRHLDL SNNSLVSLTY VSFRNLTHLE SLHLEDNALK 250 260 270 280 290 300 VLHNGTLAEL QGLPHIRVFL DNNPWVCDCH MADMVTWLKE TEVVQGKDRL TCAYPEKMRN 310 320 330 340 350 360 RVLLELNSAD LDCDPILPPS LQTSTTENLY FQGSTGTHTC PPCPAPELLG GPSVFLFPPK 370 380 390 400 410 420 PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 430 440 450 460 470 480 TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 490 500 510 520 530 540 CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 550 560 VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 62; Estimated by SDS-PAGE under reducing condition (kDa): 90-100
Calculated PI :
5.96
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 54735
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TPBG; 5T4; M6P1; 5T4AG; WAIF1; 5T4M6P1
Research Area :
Cancer
Pathway/Disease :
Wnt Signaling Pathway
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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