Nrichment. (c) KEGG pathway enrichment evaluation. to identify conserved surrounding Kcr
Nrichment. (c) KEGG pathway enrichment analysis. to identify conserved surrounding Kcr websites. We discovered that 428 zebrafish Kcr sites (76.eight ) overlapped with 339 human surrounding sequences. Furthermore, 323 human Kcr web-sites contained the exact same lysine residues as 401 Kcr web pages (72.0 ) in zebrafish. These results indicate that Kcr websites and modifications are extremely orthologous in between human and zebrafish in the protein level. Subsequent, to examine possible cross-talk/interplay between Kcr and other PTMs for example Kac and lysine ubiquitination (Kubi), Kcr results were compared with all the final results in humans using the Kac and Kubi database from PhosphoSitePlus29. In the total 323 Kcr sites converted to humans except for redundant web pages, 95 Kac (29.4 ) and 42 Kubi (13.0 ) web-sites have been detected within the similar lysine residue. Kcr is expected to be involved in PTM cross-talk/ interplay with competitive Kac rather than with Kubi. In line with the results of functional enrichment and BLASTP, Kcr in zebrafish is abundant in ribosomal proteins and myofilament proteins, such as myosin, TM and troponin. A summary of crotonylated myofilament and ribosomal proteins among zebrafish and humans is presented in Tables 1 and two, respectively. We identified 194 crotonylation internet sites in myofilament proteins in zebrafish larvae, like 156 Kcr web sites on myosin and myosinScientific REPORts | (2018) eight:3652 | DOI:ten.1038/s41598-018-22069-nature.com/scientificreports/Zebrafish Human Identities # lysine at equivalent place 82.1UniProt ID gene name # Kcr websites UniProt ID gene name protein name Q9I8U7 B8JKH7 Q6P0G6 E9QG51 O93409 F1QJP3 B8A568 B8A561 Q6IQX1 B8A569 A2BGX6 X1WF87 F1QIR4 E7FAD0 F1QJK4 E7FBZ3 P13104 Ephrin-B1/EFNB1 Protein web Q6IQD7 Q6P0W3 F1QCC0 Q0D2W2 Q6DHP2 Q6IQ92 E7EXP0 Q9I8U9 mylz3 mylz3 myl1 mylpfb mylpfa myl10 myhz1.1 myhz1.2 myhz2 myhz1.three myhc4 myhb myhz1.1 myh9b tpma tpm2 tpm3 tnni2b.1 tnni2a.four tnni2b.two tnni1al tnnt3b tnnt3a 4 4 four 1 five 2 54 57 ten 1 two 1 9 1 1 18 2 1 two 5 1 1 four three P19237 P45378 TNNI1 TNNT3 Troponin I, slow skeletal Lumican/LUM, Mouse (HEK293, His) muscle Troponin T, rapidly skeletal muscle P48788 TNNI2 Troponin I, rapid skeletal muscle P06753 TPM3 Tropomyosin alpha-3 chain Q9UKX2 Q9Y623 P35579 P09493 MYH2 MYH4 MYH9 TPM1 Myosin-2 Myosin-4 Myosin-9 Tropomyosin alpha-1 chain P12882 MYH1 Myosin-1 Q96A32 P10916 MYLPF MYL2 Myosin regulatory light chain 2, skeletal muscle isoform P14649 MYL6B Myosin light chain 6B82.5Myosin regulatory light chain 2, 82.1 ventricular/cardiac muscle isoform82.82.1 82.1 82.1 82.1 82.1 63.1 94.0 82.1 82.1 98.4 1 1 18 1 3 1zgc:171719Table 1. List of crotonylated myofilament proteins in zebrafish embryos. light chain, 22 Kcr websites on TM and 16 troponin Kcr websites, except for duplicated proteins (Table 1). Remarkably, myosin1 was higher conserved from zebrafish to human and contained 63 crotonylation internet sites in zebrafish larvae, accounting for around 11 on the total crotonylation web pages with out overlap between myhz1.1 (B8A568) and 1.two (B8A561). Interestingly, 55 crotonylation web sites in myosin had been distributed intensively inside the coiled coil motif (amino acids 841937) in comparison to the myosin motor (amino acids 8780) (Fig. four). Additionally, myosin light chain, TM and troponin have been largely conserved from zebrafish to humans and contained numerous crotonylation web-sites that are also highly equivalent to site-specific lysine residues in humans (Fig. S3). Additionally, 34 lysine sites on 21 ribosomal proteins had been crotonylated in zebrafish larvae (Table 2). Crotonylated ribosomal proteins in zebrafish had been f.
