Name :
Human CEACAM1/BGP/CD66a Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD66a, also known as CEACAM1 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 1) and BGP (biliary glycoprotein), is a single pass type I tranmembrane protein of the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPIanchored within two subgroups: the CEACAMs and the pregnancy-specific glycoproteins. In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEACAM1 contains 1 Ig-like V-type (IgV) motif and 3 Ig-like C2-type (IgC2) motifs in the N-terminal extracellular domain (ECD), followed by a transmembrane domain (TM) and an ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 has 3 soluble and 7 transmembrane isoforms due to alternate splicing. The functions of these isoforms are not clear. CEACAM1 is an adhesion molecule mediating cell adhesion via both homotypic and heterotypic associations with CEACAM5/CEA and CEACAM6/NCA. CEACAM1 is highly glycosylated and can be detected on leukocytes, epithelia, and endothelia. CEACAM1 is involved in multiple cellular processes including differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
Gene Symbol :
NCBI Gene ID :
634
Uniprot Entry :
P13688
Construct Details :
The recombinant human CEACAM1-Fc is expressed as a 632-amino acid protein consisting of Gln35 – Gly428 region of CEACAM1 (UniProt accession #P13688) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CEACAM1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QLTTESMPFN VAEGKEVLLL VHNLPQQLFG YSWYKGERVD GNRQIVGYAI GTQQATPGPA 70 80 90 100 110 120 NSGRETIYPN ASLLIQNVTQ NDTGFYTLQV IKSDLVNEEA TGQFHVYPEL PKPSISSNNS 130 140 150 160 170 180 NPVEDKDAVA FTCEPETQDT TYLWWINNQS LPVSPRLQLS NGNRTLTLLS VTRNDTGPYE 190 200 210 220 230 240 CEIQNPVSAN RSDPVTLNVT YGPDTPTISP SDTYYRPGAN LSLSCYAASN PPAQYSWLIN 250 260 270 280 290 300 GTFQQSTQEL FIPNITVNNS GSYTCHANNS VTGCNRTTVK TIIVTELSPV VAKPQIKASK 310 320 330 340 350 360 TTVTGDKDSV NLTCSTNDTG ISIRWFFKNQ SLPSSERMKL SQGNTTLSIN PVKREDAGTY 370 380 390 400 410 420 WCEVFNPISK NQSDPIMLNV NYNALPQENG LSPGSTTENL YFQGSTGTHT CPPCPAPELL 430 440 450 460 470 480 GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ 490 500 510 520 530 540 YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR 550 560 570 580 590 600 EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS 610 620 630 RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
M.W. :
Calculated molecular mass (kDa): 70; Estimated by SDS-PAGE under reducing condition (kDa): 110-120
Calculated PI :
5.70
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 94490
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Inhibits anti-CD3 antibody-induced IL-2 production in human T cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CEACAM1; CD66a; BGP; BGP1; BGPI
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD66a
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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