Name :
Human CNTFR/CNTFRA Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CNTFR or CNTFRa (Ciliary neurotrophic factor receptor subunit alpha) is a GPI-anchored surface glycoprotein of the type I cytokine receptor family, type 3 subfamily. CNTFR consists of an extracellular region with 1 Ig-like C2-type (immunoglobulin-like) domain, 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, in addition to a transmembrane (TM) domain, and a cytoplasmic tail. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding. CNTF is one of the cytokines including IL6, LIF, OSM, CNTF, IL11 and CT1 that utilize the common signaling transducer IL6ST/gp130 for initiating signal transmission. Binding of CNTF to CNTFR recruits the transmembrane components of the co-receptors, IL6ST/gp130 and leukemia inhibitory factor receptor (LIFR), facilitating signal transduction. The CNTF signaling pathway plays a critical role in neuronal cell survival, differentiation and gene expression. Single nucleotide polymorphisms in the CNTFR gene may be associated with variations in muscle strength, as well as early onset of eating disorders.
Gene Symbol :
NCBI Gene ID :
1271
Uniprot Entry :
P26992
Construct Details :
The recombinant human CNTFR-Fc protein is expressed as a 545 amino acid protein consisting of Gln23 – Glu339 region of CNTFR and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CNTFR-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QRHSPQEAPH VQYERLGSDV TLPCGTANWD AAVTWRVNGT DLAPDLLNGS QLVLHGLELG 70 80 90 100 110 120 HSGLYACFHR DSWHLRHQVL LHVGLPPREP VLSCRSNTYP KGFYCSWHLP TPTYIPNTFN 130 140 150 160 170 180 VTVLHGSKIM VCEKDPALKN RCHIRYMHLF STIKYKVSIS VSNALGHNAT AITFDEFTIV 190 200 210 220 230 240 KPDPPENVVA RPVPSNPRRL EVTWQTPSTW PDPESFPLKF FLRYRPLILD QWQHVELSDG 250 260 270 280 290 300 TAHTITDAYA GKEYIIQVAA KDNEIGTWSD WSVAAHATPW TEEPRHLTTE AQAAETTTST 310 320 330 340 350 360 TSSLAPPPTT KICDPGESTG THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 370 380 390 400 410 420 VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 430 440 450 460 470 480 VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 490 500 510 520 530 540 SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
M.W. :
Calculated molecular mass (kDa): 61.1; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
6.48
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 106060
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
GPI-anchored Protein
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Research Area :
Immunology
Pathway/Disease :
Cytokine Signaling
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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