Name :
Human LINGO1/LERN1/LRRN6A Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) is also known as LERN1 (Leucine-rich repeat neuronal protein 1) and LRRN6A (Leucine-rich repeat neuronal protein 6A). LINGO1 is a single-pass type I transmembrane protein with a 520 amino acid (aa) extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain. LINGO1 is expressed exclusively in the central nervous system (CNS) with the hghest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression can be detected. LINGO1 is up-regulated in substantia nigra neurons from Parkinson disease patients. LINGO1 is thought to be a functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation. The receptor complex is responsible for negative regulation of axonal regeneration by myelin-associated factors. LINGO1 may exist as a homotetramer and form a ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. The intracellular domain of LINGO1 interacts with MYT1L for signal transduction. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Lingo1 acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development. Inhibition of LINGO1 enhances survival, structure, and function of dopaminergic neurons in Parkinson’s disease models.
Gene Symbol :
NCBI Gene ID :
84894
Uniprot Entry :
Q96FE5
Construct Details :
The recombinant human LINGO1-Fc is expressed as a 734-amino acid protein consisting of Cys42 – Thr548 region of LINGO1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human LINGO1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 CPPRCECSAQ DRAVLCHRKR FVAVPEGIPT ETRLLDLGKN RIKTLNQDEF ASFPHLEELE 70 80 90 100 110 120 LNENIVSAVE PGAFNNLFNL RTLGLRSNRL KLIPLGVFTG LSNLTKLDIS ENKIVILLDY 130 140 150 160 170 180 MFQDLYNLKS LEVGDNDLVY ISHRAFSGLN SLEQLTLEKC NLTSIPTEAL SHLHGLIVLR 190 200 210 220 230 240 LRHLNINAIR DYSFKRLYRL KVLEISHWPY LDTMTPNCLY GLNLTSLSIT HCNLTAVPYL 250 260 270 280 290 300 AVRHLVYLRF LNLSYNPIST IEGSMLHELL RLQEIQLVGG QLAVVEPYAF RGLNYLRVLN 310 320 330 340 350 360 VSGNQLTTLE ESVFHSVGNL ETLILDSNPL ACDCRLLWVF RRRWRLNFNR QQPTCATPEF 370 380 390 400 410 420 VQGKEFKDFP DVLLPNYFTC RRARIRDRKA QQVFVDEGHT VQFVCRADGD PPPAILWLSP 430 440 450 460 470 480 RKHLVSAKSN GRLTVFPDGT LEVRYAQVQD NGTYLCIAAN AGGNDSMPAH LHVRSYSPDW 490 500 510 520 530 540 PHQPNKTFAF ISNQPGEGEA NSTRATVDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI 550 560 570 580 590 600 SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW 610 620 630 640 650 660 LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY 670 680 690 700 710 720 PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH 730 NHYTQKSLSL SPGK
M.W. :
Calculated molecular mass (kDa): 83.1; Estimated by SDS-PAGE under reducing condition (kDa): 85-90
Calculated PI :
8.02
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 87875
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
LINGO1; LERN1; LRRN6A; UNQ201; UNQ201/PRO227
Research Area :
Neuroscience
Pathway/Disease :
Neurodegenerative Diseases
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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