Name :
Human LILRB1/CD85j/ILT2/LIR1/MIR7 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
LILRB1 (Leukocyte immunoglobulin like receptor B1), also known as CD85J, LIR1 (Leukocyte immunoglobulin-like receptor 1), ILT2 (Ig-like transcript 2), and MIR7 (myeloid inhibitory receptor 7), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF). It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB1 contains 4 Ig-like C2 type domains and 4 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses. LILRB1 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected on a low percentage of T-cells and natural killer (NK) cells. LILRB1 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB1 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB1 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB1 also binds FCER1A and FCGR1A. Interaction with HLA-B or HLA-E leads to inhibition of the signal trIgGered by FCER1A and inhibits serotonin release. LILRB1 acts as a receptor for H301/UL18, a human cytomegalovirus class I MHC homolog.
Gene Symbol :
NCBI Gene ID :
10859
Uniprot Entry :
Q8NHL6
Construct Details :
The recombinant human LILRB1-Fc fusion is expressed as a 665 amino acid protein consisting of Gly24 – Val461 region of LILRB1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human LILRB1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 GHLPKPTLWA EPGSVITQGS PVTLRCQGGQ ETQEYRLYRE KKTAPWITRI PQELVKKGQF 70 80 90 100 110 120 PIPSITWEHT GRYRCYYGSD TAGRSESSDP LELVVTGAYI KPTLSAQPSP VVNSGGNVTL 130 140 150 160 170 180 QCDSQVAFDG FILCKEGEDE HPQCLNSQPH ARGSSRAIFS VGPVSPSRRW WYRCYAYDSN 190 200 210 220 230 240 SPYEWSLPSD LLELLVLGVS KKPSLSVQPG PIVAPEETLT LQCGSDAGYN RFVLYKDGER 250 260 270 280 290 300 DFLQLAGAQP QAGLSQANFT LGPVSRSYGG QYRCYGAHNL SSEWSAPSDP LDILIAGQFY 310 320 330 340 350 360 DRVSLSVQPG PTVASGENVT LLCQSQGWMQ TFLLTKEGAA DDPWRLRSTY QSQKYQAEFP 370 380 390 400 410 420 MGPVTSAHAG TYRCYGSQSS KPYLLTHPSD PLELVVSGPS GGPSSPTTGP TSTSGPEDQP 430 440 450 460 470 480 LTPTGSDPQS GLGRHLGVDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 490 500 510 520 530 540 VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 550 560 570 580 590 600 VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 610 620 630 640 650 660 SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
M.W. :
Calculated molecular mass (kDa): 73.1; Estimated by SDS-PAGE under reducing condition (kDa): 2 major bands 75-85 & 50-60 kDa probably due to proteolytic cleavage within the ECD of LILRB1
Calculated PI :
6.24
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 90730
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
LILRB1; ILT2; LIR1; MIR7; PIRB; CD85J; ILT-2; LIR-1; MIR-7; PIR-B
Research Area :
Immunology
Pathway/Disease :
MHC-I Receptor Activity
Species :
Human
CD Antigen :
CD85j
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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