Name :
Human IL21R/CD360/NILR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD360, also known as IL21R (Interleukin-21 receptor), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily. CD360/IL21R consists of an extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation. CD360/IL21R acts as a receptor for IL21 and forms a heterodimeric receptor complex with the common gamma-chain, interleukin 2 receptor gamma (IL2RG), a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. CD360/IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. IL21 binding leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies suggest a role for the IL21R/CD360 gene in regulating immunoglobulin production. The mutations affecting the IL21R/CD360 gene is associated with IL21R immunodeficiency (IL21RID), an autosomal recessive primary immunodeficiency characterized by B- and T-cell defects and variable dysfunction of NK cells. Patients have normal numbers of lymphocytes, but show defective class-switched B-cells, low IgG, defective antibody response, and defective T-cell responses to certain antigens.
Gene Symbol :
NCBI Gene ID :
50615
Uniprot Entry :
Q9HBE5
Construct Details :
The recombinant human IL21R-Fc protein is expressed as a 441 amino acid protein consisting of Cys20 – Glu232 region of IL21R and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human IL21R-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 CPDLVCYTDY LQTVICILEM WNLHPSTLTL TWQDQYEELK DEATSCSLHR SAHNATHATY 70 80 90 100 110 120 TCHMDVFHFM ADDIFSVNIT DQSGNYSQEC GSFLLAESIK PAPPFNVTVT FSGQYNISWR 130 140 150 160 170 180 SDYEDPAFYM LKGKLQYELQ YRNRGDPWAV SPRRKLISVD SRSVSLLPLE FRKDSSYELQ 190 200 210 220 230 240 VRAGPMPGSS YQGTWSEWSD PVIFQTQSEE LKESTGTHTC PPCPAPELLG GPSVFLFPPK 250 260 270 280 290 300 PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 310 320 330 340 350 360 TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 370 380 390 400 410 420 CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 430 440 VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 50.1; Estimated by SDS-PAGE under reducing condition (kDa): 55-60
Calculated PI :
5.58
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 87040
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.25 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IL21R; CD360; NILR; IL21-R
Research Area :
Immunology
Pathway/Disease :
Cytokine Signaling
Species :
Human
CD Antigen :
CD360
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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