Name :
Human CD56/NCAM1/NCAM Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
CD56, also known as NCAM1 (Neural cell adhesion molecule 1) is a single pass type I transmembrane or GPI-anchored protein of the Ig superfamily (IgSF). There are 3 main forms of human CD56 that arise by alternate splicing. CD56 contains 2 fibronectin type-III motifs and 5 Ig-like C2-type motifs in the extracellular domain (ECD). CD56 may be involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. CD56 is implicated in neural development and in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites. CD56 appears to be highly sialylated and the polysialyation reduces its adhesive property and increases its neurite outgrowth promoting features. CD56 specifically binds to heparan sulfate proteoglycans, the extracellular matrix protein agrin, and several chondroitin sulfate proteoglycans. CD56 has also been shown to be involved in the expansion of T cells, NK cells and dendritic cells which play an important role in immune surveillance. CD56 also acts as a receptor for rabies virus.
Gene Symbol :
NCBI Gene ID :
4684
Uniprot Entry :
P13591
Construct Details :
The recombinant human CD56-Fc fusion is expressed as a 926 amino acid protein consisting of Gln21 – Gly718 region of CD56 (UniProt#P13591) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD56-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QVDIVPSQGE ISVGESKFFL CQVAGDAKDK DISWFSPNGE KLTPNQQRIS VVWNDDSSST 70 80 90 100 110 120 LTIYNANIDD AGIYKCVVTG EDGSESEATV NVKIFQKLMF KNAPTPQEFR EGEDAVIVCD 130 140 150 160 170 180 VVSSLPPTII WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK KTDEGTYRCE GRILARGEIN 190 200 210 220 230 240 FKDIQVIVNV PPTIQARQNI VNATANLGQS VTLVCDAEGF PEPTMSWTKD GEQIEQEEDD 250 260 270 280 290 300 EKYIFSDDSS QLTIKKVDKN DEAEYICIAE NKAGEQDATI HLKVFAKPKI TYVENQTAME 310 320 330 340 350 360 LEEQVTLTCE ASGDPIPSIT WRTSTRNISS EEKTLDGHMV VRSHARVSSL TLKSIQYTDA 370 380 390 400 410 420 GEYICTASNT IGQDSQSMYL EVQYAPKLQG PVAVYTWEGN QVNITCEVFA YPSATISWFR 430 440 450 460 470 480 DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT AVNRIGQESL EFILVQADTP 490 500 510 520 530 540 SSPSIDQVEP YSSTAQVQFD EPEATGGVPI LKYKAEWRAV GEEVWHSKWY DAKEASMEGI 550 560 570 580 590 600 VTIVGLKPET TYAVRLAALN GKGLGEISAA SEFKTQPVQG EPSAPKLEGQ MGEDGNSIKV 610 620 630 640 650 660 NLIKQDDGGS PIRHYLVRYR ALSSEWKPEI RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ 670 680 690 700 710 720 GKSKAAHFVF RTSAQPTAIP ANGSPTSGST TENLYFQGST GTHTCPPCPA PELLGGPSVF 730 740 750 760 770 780 LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR 790 800 810 820 830 840 VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN 850 860 870 880 890 900 QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN 910 920 VFSCSVMHEA LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass (kDa): 102.8; Estimated by SDS-PAGE under reducing condition (kDa): 100-110
Calculated PI :
5.02
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 141150
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Supports the adhesion of mammalian cells such as neuroblastoma cells a dose dependent manner
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD56; NCAM1; NCAM; N-CAM-1; NCAM-1; MSK39
Research Area :
Immunology
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD56
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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