Name :
Mouse CTLA4/CD152 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
Cytotoxic T-lymphocyte antigen 4 (CTLA4), also known as CD152, is a single pass type I transmembrane glycoprotein of the Ig superfamily. It is composed of an IgV-type extracellular domain, a transmembrane domain, and a cytoplasmic tail. CTLA4 is the founding member of the CD28/CTLA-4 family. Members of the CD28/CTLA-4 family either promote T cell activation (CD28 and ICOS) or inhibit T cell activation (CTLA4 and PD1). Both CTLA-4 and CD28 bind to the same B7 family ligands, CD80/B7-1 and CD86/B7-2. CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. The CTLA4 gene is widely expressed with highest levels in lymphoid tissues. It is detected in activated T-cells where expression levels are 30- to 50-fold less than CD28 on the cell surface following activation. Mutations in CTLA4 gene is associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. Alternate transcriptional splice variants, encoding different isoforms of CTLA4, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. The engineered CTLA4-Fc fusion proteins inhibit T-cell-dependent immune responses and are used as immunosuppressive agents by acting as a competitive inhibitor of CD28. Blockade of CTLA4 inhibitory activity with monoclonal antibodies enhances antitumor immunity and has been proven to be an effective approach for cancer immunotherapy.
Gene Symbol :
Glu36 – Asp161 region of CTLA4 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
NCBI Gene ID :
12477
Uniprot Entry :
P09793
Construct Details :
The recombinant mouse CTLA4-Fc is expressed as a 354-amino acid protein consisting of Glu36 – Asp161 region of CTLA4 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Mammalian cells stably expressing mouse CTLA4-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EAIQVTQPSV VLASSHGVAS FPCEYSPSHN TDEVRVTVLR QTNDQMTEVC ATTFTEKNTV 70 80 90 100 110 120 GFLDYPFCSG TFNESRVNLT IQGLRAVDTG LYLCKVELMY PPPYFVGMGN GTQIYVIDPE 130 140 150 160 170 180 PCPDSDSTGT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV 190 200 210 220 230 240 KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE 250 260 270 280 290 300 KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT 310 320 330 340 350 TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK
M.W. :
Calculated molecular mass (kDa): 39.4; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
5.53
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 40975
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S” and “M” for marker)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CTLA4; CD152; CD; GSE; GRD4; CTLA-4; IDDM12; CELIAC3
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Mouse
CD Antigen :
CD152
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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