Name :
Mouse PD1/PD-1/CD279 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
Programmed death-1 (PD-1), also known as programmed cell death 1 (PDCD1) or CD279, is a single pass type I transmembrane glycoprotein of the Ig superfamily. It is an immunoreceptor in the CD28/CTLA-4 family with an IgV-type extracellular domain showing 21–33% sequence identity with CTLA-4, CD28 and ICOS. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or inhibit T cell activation (CTLA4 and PD1). The cytoplasmic domain of PD-1 contains two tyrosine residues, a membrane-proximal immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). ITIM is widely found in immunoinhibitory receptors and the membrane-proximal tyrosine residue may play a central role for the inhibitory function of PD-1. PD-1 negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase upon engaging with either of two ligands, PD-L1 or PD-L2. It inhibits the T-cell proliferation and production of related cytokines such as IL-1, IL-4, IL-10 and IFN-γ. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a negative regulator of T- cell effector mechanisms, PD-1 may lead to suppression of anti-tumor immunity. Blockade of PD-1 inhibitory activity with antibodies enhances anti-tumor immunity in vivo. PD-1 has been proposed as a promising target for cancer immunotherapy.
Gene Symbol :
NCBI Gene ID :
18566
Uniprot Entry :
Q02242
Construct Details :
The recombinant mouse PD1-Fc is expressed as a 376-amino acid protein consisting of Ser21 – Gly168 region of PD1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Mammalian cells stably expressing mouse PD1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 SGWLLEVPNG PWRSLTFYPA WLTVSEGANA TFTCSLSNWS EDLMLNWNRL SPSNQTEKQA 70 80 90 100 110 120 AFCNGLSQPV QDARFQIIQL PNRHDFHMNI LDTRRNDSGI YLCGAISLHP KAKIEESPGA 130 140 150 160 170 180 ELVVTERILE TSTRYPSPSP KPEGRFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL 190 200 210 220 230 240 MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ 250 260 270 280 290 300 DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG 310 320 330 340 350 360 FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA 370 LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass (kDa): 42.2; Estimated by SDS-PAGE under reducing condition (kDa): 60-65
Calculated PI :
6.56
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 67880
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S” and “M” for marker)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
PD-1; CD279, PDCD1, PD1, SLEB2, mPD1, mPD-1
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Mouse
CD Antigen :
CD279
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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