Name :
Human Nectin4/PRR4/PVRL4 Protein, Extracellular Domain (ECD), Biotinylated, Recombinant
Description :
Nectin4 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF). Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin4 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin4 and Nectin1. A soluble form of Nectin4 can be produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted Nectin4 is found in both breast tumor cell lines and breast tumor patients. Nectin4 acts as a receptor for measles virus but not for alpha-herpesvirus entry into cells. Mutations in the Nectin4 gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.
Gene Symbol :
Ser349 region of Nectin4 and a C-terminal His-tag. Nectin4 ECD contains 1 potential N-linked glycosylation sites.
NCBI Gene ID :
81607
Uniprot Entry :
Q96NY8
Construct Details :
The recombinant human Nectin4 ECD protein is expressed as a 329-amino acid active form consisting of Gly32 – Ser349 region of Nectin4 and a C-terminal His-tag. Nectin4 ECD contains 1 potential N-linked glycosylation sites.
Source :
Human cells stably expressing Nectin4 ECD and growing in chemical-defined media with no animal component or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 GELETSDVVT VVLGQDAKLP CFYRGDSGEQ VGQVAWARVD AGEGAQELAL LHSKYGLHVS 70 80 90 100 110 120 PAYEGRVEQP PPPRNPLDGS VLLRNAVQAD EGEYECRVST FPAGSFQARL RLRVLVPPLP 130 140 150 160 170 180 SLNPGPALEE GQGLTLAASC TAEGSPAPSV TWDTEVKGTT SSRSFKHSRS AAVTSEFHLV 190 200 210 220 230 240 PSRSMNGQPL TCVVSHPGLL QDQRITHILH VSFLAEASVR GLEDQNLWHI GREGAMLKCL 250 260 270 280 290 300 SEGQPPPSYN WTRLDGPLPS GVRVDGDTLG FPPLTTEHSG IYVCHVSNEF SSRDSQVTVD 310 320 VLDPQEDSGK QVDLVSASST GHHHHHHHH
M.W. :
Calculated molecular mass (kDa): 35.5; Estimated by SDS-PAGE under reducing condition (kDa): 40-45
Calculated PI :
5.57
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 31315
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
Nectin4; LNIR; PRR4; EDSS1; PVRL4; nectin-4
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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