Name :
Human ACVR1B/ALK4 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. The type I receptors are also referred to as ALKs (Activin receptor-like kinases). ACVR1B (Activin receptor type-1B), also known as ALK4, SKR2, ActR-IB, and ACVRLK4, is a type I receptor in the TGFβ receptor (TGFBR) family. ACVR1B acts as the main type I receptor to transduce signals of activin and activin-like ligands. Activin binds either ACVR2A or ACVR2B type II receptor and then forms a complex with ACVR1B to activate AVCR1B through phosphorylation. The activated ACVR1B in turn initiates downstream events by phosphorylation of its effectors to regulate gene expression and affect cellular phenotype. Activin signaling can be modulated by the direct interaction of ACVR1B with cripto or inhibin binding protein. ACVR1B plays an important role in embryonic cell fate and axis determination, cell proliferation and apoptosis, as well as tumorigenesis.
Gene Symbol :
ACVR1B; ALK4; ALK-4; SKR2; ACTRIB; ActR-IB; ACVRLK4; ACVRIB, ACVR-IB
NCBI Gene ID :
91
Uniprot Entry :
P36896
Construct Details :
The recombinant human ACVR1B-Fc fusion protein is expressed as a 331 amino acid protein consisting of Ser24 – Glu126 region of ACVR1B (UniProt accession #P36896) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as “DTT: -“)
Source :
Human cells stably expressing human ACVR1B-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
SGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPK VELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSM WGPVESTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVV VDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQD WLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKN QVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 37.0; Estimated by SDS-PAGE under reducing condition (kDa): ~45
Calculated PI :
6.31
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 47870
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized ACVR1B binds human Cripto in a functional ELISA. Blocks Cripto-mediated signaling activity.
Molecule Class :
Serine/Threonine Kinase Receptor
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
ALK4; ALK-4; SKR2; ACTRIB; ActR-IB; ACVRLK4; ACVRIB, ACVR-IB
Research Area :
Development
Pathway/Disease :
Activin Signaling Pathway
Species :
Human
CD Antigen :
References :
1. Oncogene 8:2879 (1993). 2. Proc. Natl. Acad. Sci. 91:7957 (1994). 3. Mol. Cell. Biol. 16:1066 (1996). 4. Genes Dev. 12:844 (1998). 4. Nature 404:411 (2000).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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