Name :
Human AMHR2 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. AMHR2 (Anti-Müllerian hormone type 2 receptor), also known as MRII and MISRII, is a type II receptor that forms a heteromeric complex with various type I TGFBRs to mediate AMH (anti-Müllerian hormone) or MIS (Müllerian inhibiting substance) signaling. AMH/MIS, in addition to testosterone, plays a critical role in male sex differentiation. AMH and testosterone are produced in the testes by different cells and show different effects. Testosterone promotes the development of male genitalia, while the binding of AMH/MIS to AMHR2 prevents the development of the mullerian ducts into uterus and Fallopian tubes. Mutations in the AMHR2 are associated with persistent Müllerian duct syndrome type 2 (PMDS2). AMHR2 may suppress tumorigenesis in testes.
Gene Symbol :
AMHR2; AMHR; MRII; MISR2; MISRII; MR2
NCBI Gene ID :
269
Uniprot Entry :
Q16671
Construct Details :
The recombinant human AMHR2-Fc fusion protein is expressed as a 354 amino acid protein consisting of Pro18 – Ser144 region of AMHR2 (UniProt accession #Q16671) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as “DTT: -“).
Source :
Human cells stably expressing human AMHR2-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
PPNRRTCVFFEAPGVRGSTKTLGELLDTGTELPRAIRCLYSRCCFGIWNLTQDRAQ VEMQGCRDSDEPGCESLHCDPSPRAHPSPGSTLFTCSCGTDFCNANYSHLPPPGSP GTPGSQGPQAAPGESTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTC VVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFY PSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMH EALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 39.0; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
6.40
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 44890
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Recombinant AMHR2 protein binds human AMH/MIS and blocks AMH/MIS-induced signaling activity
Molecule Class :
Serine/Threonine Kinase Receptor
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
AMHR; MRII; MISR2; MISRII; MR2
Research Area :
Development
Pathway/Disease :
AMH/MIS Signaling Pathway
Species :
Human
CD Antigen :
References :
1. Development 120:189 (1994). 2. Nat. Genet. 11:382 (1995). 3. Endocrinology 137:160 (1996). 4. Hum. Mol. Genet. 5:1269 (1996). 5. Carcinogenesis. 33:2351(2012).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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