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Name :
Mouse B7-H5 Protein, ECD (Extracellular Domain), Recombinant

Description :
B7-H5 (B7 homolog 5), also known as platelet receptor Gi24, C10orf54, Dies1, VISTA, PD-1H and SISP1, is a single-pass type I transmembrane glycoprotein that belongs to the B7 family of the Ig superfamily. Unlike other B7 family members that usually contain one Ig V-like and one Ig C-like domain in the extracellular region, mature B7-H5 has only one Ig V- like domain. B7-H5 is expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, it interacts with BMP-4 and potentiates BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, B7-H5 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. B7-H5 can be shed by MT1­MMP, generating a soluble 30 kDa extracellular fragment and a 25-30 kDa membrane-bound fragment. B7-H5 is expressed on the surface of naïve CD4+ T cells and regulatory T cells. Its expression is up­regulated in vivo on activated monocytes and dendritic cells. It is reported that B7-H5 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL­2 and IFN­γ. Its expression on tumor cells attenuates the anti­tumor immune response and enables more rapid tumor progression. In contrast, it has also been reported that B7-H5 limits disease progression in the autoimmune disease model EAE.

Gene Symbol :
The recombinant mouse B7-H5 protein is expressed as a 170 amino acid protein consisting of Phe33 – Ala191 region of mouse B7-H5 (UniProt accession #Q9D659) and and a C-terminal His-tag. It contains 3 potential sites for N-linked glycosylation.

NCBI Gene ID :
74048 (mouse)

Uniprot Entry :
Q9D659 (mouse)

Construct Details :
The recombinant mouse B7-H5 protein is expressed as a 170 amino acid protein consisting of Phe33 – Ala191 region of mouse B7-H5 (UniProt accession #Q9D659) and and a C-terminal His-tag. It contains 3 potential sites for N-linked glycosylation.

Source :
Human cells stably expressing mouse B7-H5 ECD and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 FKVTTPYSLY VCPEGQNATL TCRILGPVSK GHDVTIYKTW YLSSRGEVQM CKEHRPIRNF 70 80 90 100 110 120 TLQHLQHHGS HLKANASHDQ PQKHGLELAS DHHGNFSITL RNVTPRDSGL YCCLVIELKN 130 140 150 160 170 HHPEQRFYGS MELQVQAGKG SGSTCMASNE QDSDSITAAS TGHHHHHHHH

M.W. :
Calculated molecular mass 19.1 kDa; estimated by SDS-PAGE under reducing condition 40-45 kDa probably due to glycosylation

Calculated PI :
7.89

Calculated Extinction Coefficients :

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Inhibits anti­-CD3e antibody induced IL­2 secretion and T cell proliferation.

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
B7-H5; B7H5; Gi24; Sisp-1; SISP1;C10orf54; VISTA; Dies1; PD-1H; PP2135; UNQ730/PRO1412

Research Area :
Immunology

Pathway/Disease :
T Cell Costimulation

Species :
Mouse

CD Antigen :

References :

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