Name :
Human GITR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
GITR (Glucocorticoid-induced TNFR-related protein), also known as CD357 and AITR (Activation-inducible TNFR family receptor), is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF18). GITR contains 3 TNFR cysteine-rich repeats in the extracellular region and a cytoplasmic domain that shows striking homology with those of TNFRSF members 41BB/CD137 and CD27. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. GITR is up regulated in peripherical mononuclear cells after antigen stimulation/lymphocyte activation by treatment with antiCD3 plus antiCD28, or PMA plus ionomycin. Knockout studies indicate that GITR may play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells and in the regulation of CD3-driven T-cell activation and programmed cell death. GITR is the receptor for GITR ligand (GITRL) belonging to the TNF superfamily (TNFSF18). GITR and its ligand GITRL are involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Ligation of GITRL can activate NFκB through several TRAF adaptor proteins, and protect T cells from TCR activationinduced cell death. GITR and GITRL have also been shown to be important co-stimulatory molecules in the pathogenesis of autoimmune diseases.
Gene Symbol :
TNFRSF18; AITR; CD357; GITR-D; UNQ319/PRO364
NCBI Gene ID :
8784
Uniprot Entry :
Q9Y5U5
Construct Details :
The recombinant human GITR-Fc fusion is expressed as a 364 amino acid protein consisting of Gln26 – Glu161 region of GITR (UniProt accession #Q9Y5U5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human GITR-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC 70 80 90 100 110 120 CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG 130 140 150 160 170 180 NKTHNAVCVP GSPPAESTGT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV 190 200 210 220 230 240 VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV 250 260 270 280 290 300 SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES 310 320 330 340 350 360 NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK
M.W. :
Calculated molecular mass (kDa): 40.1; Estimated by SDS-PAGE under reducing condition (kDa): 45-50
Calculated PI :
6.72
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 49400
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand GITRL/TNFSF18 and blocks GITRL-induced signaling activity.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TNFRSF18; AITR; CD357; GITR-D; UNQ319/PRO364
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD357
References :
1. Proc. Natl. Acad. Sci. USA 94:6216 (1997). 2. J. Biol. Chem. 274:6056 (1999). 3. Current Biology 9:215 (1999). 4. Cell Death Differ. 7:408 (2000). 5. Oncol Rep. 18: 1189 (2007).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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