Name :
Human BAFFR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
BAFFR (B- cell-activating factor receptor), also known as CD268 and BR3 (BLyS receptor 3), is a single-pass type III transmembrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF13C). BAFFR contains only one partial TNFR cysteine-rich repeat with 4 cysteine residues in the extracellular region but lacks a cytoplasmic death domain (DD). BAFFR is highly expressed in spleen and lymph node, and in resting B-cells. BAFFR is detected at lower levels in activated B-cells, resting T-cells, in thymus and peripheral blood leukocytes. BAFFR is a B-cell receptor specific for the TNF superfamily ligand, BAFF /TNFSF13B. BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to3 TNFRSF members: BCMA (Bcell maturation antigen – TNFRSF17), TACI (transmembrane activator and calciummodulator and cyclophilin ligand interactor -TNFRSF13B) and BAFFR. TACI and BCMA bind BAFF and another TNFSF ligand, APRIL (a proliferationinducing ligand). In contrast, BAFFR selectively binds BAFF. BAFF knockout mice lack mature B cells while BCMA or TACIdeficient mice have no major defect in Bcell development. Mutations in the BAFFR gene are associated with immunodeficiency common variable type 4 (CVID4), which is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. CVID4 results from a failure of B-cell differentiation and impaired secretion of immunoglobulins.
Gene Symbol :
TNFRSF13C; CD268; BR3; CVID4; BAFF-R; BROMIX; prolixin
NCBI Gene ID :
115650
Uniprot Entry :
Q96RJ3
Construct Details :
The recombinant human BAFFR-Fc fusion is expressed as a 300 amino acid protein consisting of Arg2 – Gly76 region of BAFFR (UniProt accession #Q96RJ3) and a C-terminal Fc from human IgG1, which exists as a dimer/tetramer/oligomer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human BAFFR-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 RRGPRSLRGR DAPAPTPCVP AECFDLLVRH CVACGLLRTP RPKPAGASSP APRTALQPQE 70 80 90 100 110 120 SVGAGAGEAA LPLPGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS 130 140 150 160 170 180 HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA 190 200 210 220 230 240 LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP 250 260 270 280 290 300 ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 33; Estimated by SDS-PAGE under reducing condition (kDa): 40-45
Calculated PI :
8.62
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 36035
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand BAFF. Inhibits BAFF-mediated signaling activities including NF-κB activation and proliferation of anti-IgM co-stimulated B cells.
Molecule Class :
1-Pass Type III Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
BAFFR; TNFRSF13C; CD268; BR3; CVID4; BAFF-R; BROMIX; prolixin
Research Area :
Immunology
Pathway/Disease :
B Cell Development
Species :
Human
CD Antigen :
CD268
References :
1. Science 14:2108 (2001). 2. Current Biol. 11:R1013 (2001). 3. Nat. Rev. Immunol. 2:464 (2002). 4. Curr. Opin. Immunol. 14:266 (2002). 5. Adv. Cancer Res.86: 195 (2003).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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