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Name :
Human BTLA/CD272 protein, ECD (extracellular domain), recombinant

Description :
CD272, also known as BTLA (B- and T-lymphocyte attenuator), is a single pass type I transmembrane glycoprotein that belongs to the CD28/CTLA4 family of the Ig superfamily. Like CD28 and CTLA4, CD272 contains one Ig-like domain in the extracellular region. However, the Ig-like domain is an I­type rather than a V­type domain commonly found in other family members. CD272 also differs from other CD28/CTLA4 family members through the interaction of its Ig-like domain with the TNF superfamily member, HVEM (TNFRSF14) rather than with B7 family ligands. CD272 is an immune co-inhibitory molecule expressed on T cells, B cells and, macrophages, dendritic and NK cells. The HVEM-CD272 ligation relays inhibitory signals to suppress the immune response. Expression of CD272 is low in naïve T cells and increased during antigen­specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve. BTLA appears to limit T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8+ memory T cells in mice. There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail of CD272 that mediate inhibitory signaling. Polymorphisms in the CD272 gene have been associated with an increased risk of rheumatoid arthritis.

Gene Symbol :
The recombinant human BTLA/CD272 ECD protein is expressed as a 133 amino acid protein consisting of Lys31 – Pro152 region of CD272 (UniProt accession #Q7Z6A9) and and a C-terminal His-tag. It contains 3 potential sites for N-linked glycosylation.

NCBI Gene ID :
151888

Uniprot Entry :
Q7Z6A9

Construct Details :
The recombinant human BTLA/CD272 ECD protein is expressed as a 133 amino acid protein consisting of Lys31 – Pro152 region of CD272 (UniProt accession #Q7Z6A9) and and a C-terminal His-tag. It contains 3 potential sites for N-linked glycosylation.

Source :
Human cells stably expressing CD272 ECD and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 KESCDVQLYI KRQSEHSILA GDPFELECPV KYCANRPHVT WCKLNGTTCV KLEDRQTSWK 70 80 90 100 110 120 EEKNISFFIL HFEPVLPNDN GSYRCSANFQ SNLIESHSTT LYVTDVKSAS ERPSKDEMAS 130 RPSTGHHHHH HHH

M.W. :
Calculated molecular mass 15.3 kDa; estimated by SDS-PAGE under reducing condition 30-40 kDa probably due to glycosylation

Calculated PI :
6.66

Calculated Extinction Coefficients :

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free)

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds human HVEM/TNFRSF14 and blocks HVEM-CD272 mediated signaling activity.

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
BTLA; CD272; BTLA1; BTLA-1

Research Area :
Immunology

Pathway/Disease :
T Cell Costimulation

Species :
Human

CD Antigen :
CD272

References :

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