Name :
Human CD40 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD40 s a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF5). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. CD40 plays an essential role in mediating a broad range of immune and inflammatory responses including immunoglobulin class switching, B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular region. Interaction of CD40 with its ligand, CD40 Ligand (CD40L, also known as CD154), leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells. CD40 ligation by CD40L promotes B cell activation and T celldependent humoral responses. Mutations in CD40 are associated with hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching or to mount an antibody-specific immune response. CD40 is also implicated in the pathology of multiple cardiovascular diseases, such as atherosclerosis, atherothrombosis, and restenosis. CD40/CD40L interaction may be required for amyloid-beta-induced microglial activation, an early event in Alzheimer disease pathogenesis. In addition CD40 plays important roles in both hematopoietic and epithelial cancers. CD40 has been a target for tumor immunotherapy.
Gene Symbol :
p50; Bp50; CDW40; CD40LR; TNFRSF5
NCBI Gene ID :
958
Uniprot Entry :
P25942
Construct Details :
The recombinant human CD40-Fc fusion is expressed as a 401 amino acid protein consisting of Glu21 – Arg193 region of CD40 (UniProt accession #P25942) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human CD40-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EPPTACREKQ YLINSQCCSL CQPGQKLVSD CTEFTETECL PCGESEFLDT WNRETHCHQH 70 80 90 100 110 120 KYCDPNLGLR VQQKGTSETD TICTCEEGWH CTSEACESCV LHRSCSPGFG VKQIATGVSD 130 140 150 160 170 180 TICEPCPVGF FSNVSSAFEK CHPWTSCETK DLVVQQAGTN KTDVVCGPQD RLRSTGTHTC 190 200 210 220 230 240 PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN 250 260 270 280 290 300 AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP 310 320 330 340 350 360 QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL 370 380 390 400 YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 44.7; Estimated by SDS-PAGE under reducing condition (kDa): 55-60
Calculated PI :
5.75
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 56515
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand CD40L/CD154 and inhibits CD40L-induced signaling activity including NF-κB activation and proliferation of human B cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD40; p50; Bp50; CDW40; CD40LR; TNFRSF5
Research Area :
Immunology
Pathway/Disease :
B Cell Development
Species :
Human
CD Antigen :
CD40
References :
1. EMBO J. 8:1403 (1989). 2. Annu. Rev. Immunol. 12:881 (1994). 3. J. Biol. Chem. 272:19569 (1997). 4. Proc. Natl. Acad. Sci. 97: 10395 (2000). 5. Immunol. Rev. 244:115 (2011).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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