Name :
Mouse PD-L1 protein, ECD (extracellular domain), Fc-fusion, recombinant
Description :
PD-L1 (programmed cell death 1 ligand 1) or CD274 is a 290-amino acid single pass type I transmembrane protein of the Ig (immunoglobulin) superfamily. It contains one Ig V-like and one Ig C-like domains in the extracellular region, and a 30-amino acid cytoplasmic tail. It was originally cloned as a homolog of B7 (termed B7-H1). It is 20% and 15% identical to B7-1 (CD80) and B7-2 (CD86), respectively. It was also cloned as a ligand (termed PDCD1L1 or PD-L1) that binds to PD-1 (CD279) and B7-1 (CD80), but not to other B7 family members, such as CTLA4 (CD152), CD28, or ICOS (CD278). PD-L1 or CD274 is involved in the T cell costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNγ. Interaction with PD-1, which has a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), inhibits T-cell proliferation and cytokine production. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNγ activation or by surface Ig cross-linking. It is expressed in many types of freshly isolated human cancers but not in most normal tissues. Blockade of PD-L1 upregulates IL12 expression and enhances antitumor immunity in mice bearing ovarian tumors. Therefore, PD-L1 has been proposed as a target for cancer immunotherapy through pre-activated T cells that could be enhanced by blockade of PD-L1-induced inhibitory signaling.
Gene Symbol :
The recombinant mouse PD-L1/CD274 ECD is expressed as a 458 amino acid protein consisting of Phe19 – Thr238 region of mouse PD-L1/CD274 (UniProt accession #Q9EP73) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
NCBI Gene ID :
60533
Uniprot Entry :
Q9EP73
Construct Details :
The recombinant mouse PD-L1/CD274 ECD is expressed as a 458 amino acid protein consisting of Phe19 – Thr238 region of mouse PD-L1/CD274 (UniProt accession #Q9EP73) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Mammalian cells stably expressing mouse PD-L1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 FTITAPKDLY VVEYGSNVTM ECRFPVEREL DLLALVVYWE KEDEQVIQFV AGEEDLKPQH 70 80 90 100 110 120 SNFRGRASLP KDQLLKGNAA LQITDVKLQD AGVYCCIISY GGADYKRITL KVNAPYRKIN 130 140 150 160 170 180 QRISVDPATS EHELICQAEG YPEAEVIWTN SDHQPVSGKR SVTTSRTEGM LLNVTSSLRV 190 200 210 220 230 240 NATANDVFYC TFWRSQPGQN HTAELIIPEL PATHPPQNRT STTENLYFQG STGTHTCPPC 250 260 270 280 290 300 PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT 310 320 330 340 350 360 KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY 370 380 390 400 410 420 TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK 430 440 450 LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated PI :
6.00
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its receptor PD-1 and anti-PD-L1 monoclonal antibody (SKU#: MAB0199, MAB0784, MAB0781 – see Technical Data). Blocks PD-L1’s binding to its receptor and mediated signaling activity. Shows cross-species binding to human PD-1 (SKU#FCL0763) and human CD80 (SKU#FCL0723) in a functional ELISA (see Technical Data).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
PD-L1, PDCD1LG1, CD274, B7-H1, B7H1, PDL1, PDCD1L1
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Mouse
CD Antigen :
CD274
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
EDNRA Protein
CBFB Protein
Popular categories:
Cadherin-15
MIP-3 beta/CCL19
