Name :
Human DcR1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
DcR1 ( decoy receptor 1), also known as CD263, TRAILR3, LIT and TRID, is a glycosylphosphatidylinositol (GPI)linked membrane protein of the TNF receptor superfamily (TNFRSF10C). DcR1 is highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart. DcR1 is one of 5 receptors for TRAIL (tumor necrosis factor-related apoptosis inducing ligand), also known as Apo2L (Apo-2 ligand) and TNFSF10. TRAIL preferentially induces apoptosis in tumor cell lines, but not in normal cells. In addition to DcR2, DcR3 and OPG, DcR1 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. DcR1 binds TRAIL with high affinity. However DcR1 lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. DcR1 may protect cells against TRAIL mediated apoptosis by competing with DR4/TRAILR1 and DR5/TRAILR2 for binding to the ligand. Expression of DcR1 has been shown to protect cells bearing DR4 and/or DR5 from TRAILinduced apoptosis. DcR1 is also a p53-regulated DNA damage-inducible gene. DcR1 is detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL. The soluble DcR1-Fc fusion protein neutralizes the ability of TRAIL to induce apoptosis.
Gene Symbol :
DcR1; LIT; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR; TNFRSF10C
NCBI Gene ID :
8794
Uniprot Entry :
O14798
Construct Details :
The recombinant human DcR1-Fc fusion is expressed as a 438 amino acid protein consisting of Ala26 – Pro235 region of DcR1 (UniProt accession #O14798) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).
Source :
Human cells stably expressing human DcR1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ATTARQEEVP QQTVAPQQQR HSFKGEECPA GSHRSEHTGA CNPCTEGVDY TNASNNEPSC 70 80 90 100 110 120 FPCTVCKSDQ KHKSSCTMTR DTVCQCKEGT FRNENSPEMC RKCSRCPSGE VQVSNCTSWD 130 140 150 160 170 180 DIQCVEEFGA NATVETPAAE ETMNTSPGTP APAAEETMNT SPGTPAPAAE ETMTTSPGTP 190 200 210 220 230 240 APAAEETMTT SPGTPAPAAE ETMITSPGTP STGTHTCPPC PAPELLGGPS VFLFPPKPKD 250 260 270 280 290 300 TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL 310 320 330 340 350 360 HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV 370 380 390 400 410 420 KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH 430 EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 47.7; Estimated by SDS-PAGE under reducing condition (kDa): 65-75
Calculated PI :
5.22
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 43650
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand human TRAIL and inhibits TRAIL-mediated signaling activity including TRAILinduced cytotoxicity in L-929 mouse fibroblast cells.
Molecule Class :
GPI-anchored Protein
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
DcR1; LIT; TRID; CD263; TRAILR3; TRAIL-R3; DCR1-TNFR; TNFRSF10C
Research Area :
Immunology
Pathway/Disease :
Apoptosis
Species :
Human
CD Antigen :
CD263
References :
1. FEBS Lett. 416:329-334(1997). 2. J. Biol. Chem. 272:25417 (1997). 3. J. Exp. Med. 186:1165 (1997). 4. Science 277:815 (1997). 5. Science 277:818 (1997). 6. J. Immunol. 160:3 (1998).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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