Name :
Mouse Fc (IgG2a) Control Protein, Biotinylated, Recombinant
Description :
The mus musculus or mouse IGHG (immunoglobulin heavy constant gamma) gene encodes the Immunoglobulin (Ig) heavy chain gamma polypeptide or Ig gamma-2A chain C region, A allele. The C region contains 3 Ig-like domains including the CH1, hinge, CH2 and CH3 domains. The hinge, CH2 and CH3 region is also commonly referred to as the Fc region. Fc meditates the interactions with the complement components and Fc-gamma receptors (FCGRs) to induce innate immune responses such as antibody-dependent complement activation and phagocytosis, respectively. The mouse Fc control protein is expressed as a recombinant protein that include the hinge , CH2 and CH3 domains of mouse Ig gamma-2a chain C region (which can be used for making mouse Fc-fusion proteins). The purified protein can be used as a negative control for mouse Fc-fusion proteins in a variety of biological assays.
Gene Symbol :
IGHG; IGHG2A; AU044919
NCBI Gene ID :
380794
Uniprot Entry :
P01863
Construct Details :
The recombinant mouse Fc control protein is expressed as a 233 amino acid protein consisting of Glu98 – Lys330 region of mouse IGHG or Ig gamma-2A chain C region, A allele (UniProt accession #P01863), which exists as a dimer under non-reducing condition (see the gel image inserted).
Source :
Human cells stably expressing mouse Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EPRGPTIKPC PPCKCPAPNL LGGPSVFIFP PKIKDVLMIS LSPIVTCVVV DVSEDDPDVQ 70 80 90 100 110 120 ISWFVNNVEV HTAQTQTHRE DYNSTLRVVS ALPIQHQDWM SGKEFKCKVN NKDLPAPIER 130 140 150 160 170 180 TISKPKGSVR APQVYVLPPP EEEMTKKQVT LTCMVTDFMP EDIYVEWTNN GKTELNYKNT 190 200 210 220 230 EPVLDSDGSY FMYSKLRVEK KNWVERNSYS CSVVHEGLHN HHTTKSFSRT PGK
M.W. :
Calculated molecular mass 26.4 kDa; estimated by SDS-PAGE under reducing condition 30-35 kDa probably due to glycosylation
Calculated PI :
6.74
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
Ig (secreted)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IGHG; IGHG2A; AU044919
Research Area :
Immunology
Pathway/Disease :
FCGR Signaling Pathway
Species :
Mouse
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
ULBP4/RAET1E Protein
OSM Protein
Popular categories:
B7-H6
CEA Cell Adhesion Molecule 21
