Name :
Rabbit Fc (IgG) Control Protein, Recombinant
Description :
The oryctolagus cuniculus or rabbit IGHG (immunoglobulin heavy constant gamma) gene encodes the Ig gamma (IgG) chain constant (C) region. The C region contains 3 Ig-like domains including the CH1, hinge, CH2 and CH3 domains. The hinge, CH2 and CH3 region is also commonly referred to as the Fc region. Fc meditates the interactions with the complement components and Fc-gamma receptors (FCGRs) to induce innate immune responses such as antibody-dependent complement activation and phagocytosis, respectively. The rabbit Fc control protein is expressed as a recombinant protein that include the hinge , CH2 and CH3 domains of rabbit IgG C region (which can be used for making rabbit Fc-fusion proteins). The purified protein can be used as a negative control for rabbit Fc-fusion proteins in a variety of biological assays.
Gene Symbol :
IGHG
NCBI Gene ID :
Uniprot Entry :
P01870
Construct Details :
The recombinant rabbit Fc control protein is expressed as a 229 amino acid protein consisting of Val95 – Lys323 region of rabbit IGHG or Ig gamma chain C region (UniProt accession #P01870), which exists as a dimer under non-reducing condition.
Source :
Mammalian cells stably expressing rabbit Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 VAPSTCSKPT CPPPELLGGP SVFIFPPKPK DTLMISRTPE VTCVVVDVSQ DDPEVQFTWY 70 80 90 100 110 120 INNEQVRTAR PPLREQQFNS TIRVVSTLPI THQDWLRGKE FKCKVHNKAL PAPIEKTISK 130 140 150 160 170 180 ARGQPLEPKV YTMGPPREEL SSRSVSLTCM INGFYPSDIS VEWEKNGKAE DNYKTTPAVL 190 200 210 220 DSDGSYFLYN KLSVPTSEWQ RGDVFTCSVM HEALHNHYTQ KSISRSPGK
M.W. :
Calculated molecular mass 25.8 kDa; estimated by SDS-PAGE under reducing condition 30-35 kDa probably due to glycosylation
Calculated PI :
8.20
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
Ig (secreted)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IGHG; IGHG2A; AU044919
Research Area :
Immunology
Pathway/Disease :
FCGR Signaling Pathway
Species :
Rabbit
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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