Name :
Human OPG Protein, Fc-fusion, Recombinant
Description :
OPG (Osteoprotegerin), also known as OCIF (osteoclastogenesis inhibitory factor), is a secreted 55 – 60 kDa protein of the TNF-receptor superfamily (TNFRSF11B). Mature OPG is a 380 amino acid soluble protein consisting of 4 TNFR cysteine-rich repeats, 2 death domains (DD) and 1 heparin-binding region. The cysteine-rich repeats are essential for ligand interaction, while a cysteine at the C-terminus mediates homodimerization. OPG is widely expressed and constitutively released by mesenchymal stem cells, fibroblasts and endothelial cells. OPG is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. OPG specifically binds to its ligand, OPG ligand or RANKL (receptor activator of NF-κB ligand), both of which are key extracellular regulators of osteoclast development. OPG and RANKL may also play a role in lymph-node organogenesis and vascular calcification. OPG regulates bone density through inhibiting the activation of osteoclasts and promoting osteoclast apoptosis. Bone homeostasis may depend on the local RANKL to OPG ratio. OPG also act as a decoy receptor for TRAIL (TNF-related apoptosis-inducing ligand). TRAIL binding blocks the inhibition of osteoclastogenesis. In addition TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL-induced apoptosis. Binding of OPG by syndecan-1 heparin sulfates on multiple myeloma cells also results in OPG internalization and degradation, contributing to bone loss. OPG deficiency can cause juvenile Paget’s disease (JPD) in humans, and insufficient OPG can produce osteoporosis and vascular calcification in animals and humans.
Gene Symbol :
OPG; TR1; OCIF; TNFRSF11B
NCBI Gene ID :
4982
Uniprot Entry :
O00300
Construct Details :
The recombinant human OPG-Fc fusion is expressed as a 608 amino acid protein consisting of Glu22 – Leu401 region of OPG (UniProt accession #O00300) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human OPG-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL 70 80 90 100 110 120 YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV 130 140 150 160 170 180 CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQKCGIDVTL 190 200 210 220 230 240 CEEAFFRFAV PTKFTPNWLS VLVDNLPGTK VNAESVERIK RQHSSQEQTF QLLKLWKHQN 250 260 270 280 290 300 KDQDIVKKII QDIDLCENSV QRHIGHANLT FEQLRSLMES LPGKKVGAED IEKTIKACKP 310 320 330 340 350 360 SDQILKLLSL WRIKNGDQDT LKGLMHALKH SKTYHFPKTV TQSLKKTIRF LHSFTMYKLY 370 380 390 400 410 420 QKLFLEMIGN QVQSVKISCL STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV 430 440 450 460 470 480 TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY 490 500 510 520 530 540 KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV 550 560 570 580 590 600 EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 69.2; Estimated by SDS-PAGE under reducing condition (kDa): 75-85
Calculated PI :
8.55
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 81050
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligands TRAIL and RANKL. Inhibits TRAIL- and RANKL-mediated signaling activity including NF-kB activation and TRAILinduced cytotoxicity in L-929 mouse fibroblast cells.
Molecule Class :
Decoy Receptor (secreted)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
OPG; TR1; OCIF; TNFRSF11B
Research Area :
Metabolism
Pathway/Disease :
Osteoclastogenesis
Species :
Human
CD Antigen :
References :
1. Cell 89:309 (1997). 2. Proc. Natl. Acad. Sci. 95:3597 (1998). 3. J. Biol. Chem. 273:5117 (1998). 4. N. Engl. J. Med. 347:175 (2002). 5. Bone 39:706 (2006). 6. J. Atherosclerosis 204:321 (2009).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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