Name :
Human TACI Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
TACI (transmembrane activator and CAMLinteractor), also known as CD267, is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF13B). The TNFRSF members have crucial roles in both innate and adaptive immunity as well as in apoptosis. The extracellular region of TACI contains two TNFR-cysteine rich repeats that mediate interactions with its TNF family ligands, including APRIL/TNFSF13 and BAFF/TNFSF13B. TACI binds both ligands with similar high affinity. Within the TNFRSF, it shares the highest homology with BCMA/TNFRSF17. TACI and BCMA can bind both APRIL and BAFF. TACI is expressed on the cell surface of B cells and activated T cells, but not on resting T cells. TACI may play an important role in B cell development, function and regulation. TACI interacts with calcium-modulator and cyclophilin ligand (CAML). TACI mediates calcineurin-dependent activation of NF-AT, NF-κB and AP-1. TACI is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. Mutations in the TACI gene are associated with common variable immunodeficiency 2 (CVID2) and immunoglobulin A deficiency 2 (IGAD2) in humans.
Gene Symbol :
TACI; CD267; CVID; RYZN; CVID2; TNFRSF13B
NCBI Gene ID :
23495
Uniprot Entry :
O14836
Construct Details :
The recombinant human TACI-Fc fusion is expressed as a 391 amino acid protein consisting of Ser2 – Ser165 region of TACI (UniProt accession #O14836) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human TACI-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 SGLGRSRRGG RSRVDQEERF PQGLWTGVAM RSCPEEQYWD PLLGTCMSCK TICNHQSQRT 70 80 90 100 110 120 CAAFCRSLSC RKEQGKFYDH LLRDCISCAS ICGQHPKQCA YFCENKLRSP VNLPPELRRQ 130 140 150 160 170 180 RSGEVENNSD NSGRYQGLEH RGSEASPALP GLKLSADQVA LVYSTGTHTC PPCPAPELLG 190 200 210 220 230 240 GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY 250 260 270 280 290 300 NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE 310 320 330 340 350 360 EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR 370 380 390 WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 43.9; Estimated by SDS-PAGE under reducing condition (kDa): 45-50
Calculated PI :
8.45
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 54985
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand BAFF. Inhibits BAFF-mediated signaling activities including NF-κB activation and proliferation of anti-IgM co-stimulated B cells.
Molecule Class :
1-Pass Type III Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TACI; CD267; CVID; RYZN; CVID2; TNFRSF13B
Research Area :
Immunology
Pathway/Disease :
B Cell Development
Species :
Human
CD Antigen :
CD267
References :
1. Science, 278:138 (1997). 2. J. Exp. Med. 192:137 (2000). 3. Nature 404:995 (2000). 4. Nature Immunol.1:37 (2000). 5. Nat Genet. 37: 820 (2005)
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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