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Name :
Human HER2 protein (extracellular domain), recombinant

Description :
HER2 is also known as receptor tyrosine-protein kinase erbB-2 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 2), metastatic lymph node gene 19 protein, proto-oncogene Neu, p185erbB2. It is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases (RTK). Four members of the EGFR family have been identified: EGFR (ERBB1, HER1), HER2 (ERBB2), HER3 (ERBB3) and HER4 (ERBB4). They typically contain an extracellular ligand binding domain (ECD), a transmembrane domain (TM), and an intracellular kinase domain that can interact with a multitude of signaling molecules and exhibit both ligand-dependent and ligand-independent activity. EGFR signaling is initiated by ligand binding to the extracellular ligand binding domain. This initiates receptor homo-/hetero-dimerization and autophosphorylation by the intracellular kinase domain, resulting in receptor activation. Following activation, phosphorylation of cytoplasmic substrates occurs and a signaling cascade is initiated that drives many cellular responses, including changes in gene expression, cytoskeletal rearrangement, anti-apoptosis and increased cell proliferation. HER2 has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGFR family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways. Amplification or overexpression of HER2 has been reported in multiple cancers, including breast and ovarian tumors. HER2 is the target of several anti-cancer therapeutics.

Gene Symbol :

NCBI Gene ID :

Uniprot Entry :

Construct Details :
The recombinant human HER2 extracellular or ecto-domain (ECD) is expressed as a 630 amino acid protein consisting of Thr23-Thr652 region of HER2 and a C-terminal poly-Histidine tag, which exists as a monomer

Source :
Human cells stably expressing HER2 ECD and growing in chemical-defined media with no animal components or antibiotics.

Amino Acid Sequence: :

M.W. :
Calculated molecular mass (kDa): 70.7; Estimated by SDS-PAGE under reducing condition (kDa): ~75 (see the gel image) suggesting that the protein is glycosylated

Calculated PI :

Calculated Extinction Coefficients :

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
The recombinant protein binds anti-HER2 mAbs (SKU#MAB0061, #MAB0203, #MAB0334) with high affinity (KD and therapeutic antibodies such as Herceptin® with high affinity (KD

Molecule Class :
Receptor Tyrosine Kinase (RTK)

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
HER2; ERBB2; CD340; NGL; TKR1; MLN19; HER-2/neu

Research Area :

Pathway/Disease :
EGF/PDGF Signaling Pathway

Species :

CD Antigen :

References :
1. Science 230: 1132–9, 1985

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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