Name :
Human ACVR1/ALK2 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. The type I receptors are also referred to as ALKs (Activin receptor-like kinases). ACVR1 (Activin receptor type-1), also known as ACVR1A, ALK2, FOP, SKR1, TSRI, ACVRLK2, and ActRI, is a type I receptor in the TGFβ receptor family. ACVR1 was identified as a receptor for both Activin and BMP. It is expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. ACVR1 plays a critical role in gastrulation, skeletal system development, and cardiac morphogenesis. It may be required for left-right pattern formation during embryogenesis. Overexpression of ACVR1 causes a mitotic arrest. ACVR1 can phosphorylate the co-receptor Endoglin and mediate the inhibitory effect of Endoglin on prostate cancer cell motility. Defects in ACVR1 are associated with fibrodysplasia ossificans progressiva (FOP).
Gene Symbol :
ACVR1; ALK2; FOP; ALK-2; SKR1; TSRI; TSR-I; ACTRI; ActR-I; ACVR1A; ACVRLK2; ACVRIA; ACVR-IA
NCBI Gene ID :
90
Uniprot Entry :
Q04771
Construct Details :
The recombinant human ACVR1-Fc fusion protein is expressed as a 331amino acid protein consisting of Met21 – Glu123 region of ACVR1 (UniProt accession #Q04771) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as “DTT: -“).
Source :
Human cells stably expressing human ACVR1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
MEDEKPKVNPKLYMCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGC FQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNF HLESTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDV SHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNG KEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 37.1; Estimated by SDS-PAGE under reducing condition (kDa): ~45
Calculated PI :
6.37
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 46380
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized ACVR1 protein binds human BMP6 in a functional ELISA. Blocks BMP6-mediated signaling activity.
Molecule Class :
Serine/Threonine Kinase Receptor
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
ALK2; FOP; ALK-2; SKR1; TSRI; TSR-I; ACTRI; ActR-I; ACVR1A; ACVRLK2; ACVRIA; ACVR-IA
Research Area :
Development
Pathway/Disease :
Activin/BMP Signaling Pathway
Species :
Human
CD Antigen :
References :
1. Cell 75:671 (1993). 2. J. Biol. Chem. 268:12719 (1993). 3. Oncogene 8:2879 (1993). 4. Genes Dev. 18:1838 (2004). 5. Nat. Genet. 38:525 (2006).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CXCL16 Protein
IFN-omega Protein
Popular categories:
Junctional Adhesion Molecule B (JAM-B)
Tissue Inhibitor of Metalloproteinase 4 (TIMP-4)
