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Name :
Human CSF1/MCSF Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description :
CSF1 (colony stimulating factor 1), also known as M­CSF (macrophage colony stimulating factor) is a single-pass type I transmembrane or secreted protein. The active form of CSF1 is a disulfide-linked homodimer, produced by proteolytic cleavage of membrane-bound precursors. As a cytokine, CSF1 controls the production, differentiation, and function of macrophages. CSF1 primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. CSF1 induces the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. CSF1 is required for male and female fertility. CSF1 induces reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Aberrant expression of CSF1 or its receptor CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in many types of cancers including breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R is implicated in inflammatory conditions, such as rheumatoid arthritis (RA), glomerulonephritis, atherosclerosis, and allograft rejection.

Gene Symbol :
CSF1; MCSF; CSF-1; M-CSF

NCBI Gene ID :
1435

Uniprot Entry :
P09603

Construct Details :
The recombinant human CSF1-Fc is expressed as a 388-amino acid protein consisting of Glu33 – Gln181 region of CSF1 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.

Source :
Human cells stably expressing human CSF1-Fc and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI 70 80 90 100 110 120 MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV 130 140 150 160 170 180 FNETKNLLDK DWNIFSKNCN NSFAECSSQG STTENLYFQG STGTHTCPPC PAPELLGGPS 190 200 210 220 230 240 VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST 250 260 270 280 290 300 YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT 310 320 330 340 350 360 KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ 370 380 GNVFSCSVMH EALHNHYTQK SLSLSPGK

M.W. :
Calculated molecular mass (kDa): 44.2 Estimated by SDS-PAGE under reducing condition (kDa): 50-55.

Calculated PI :
5.66

Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 49110

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds its receptor human CSF1R (SKU#FCL2648) and induces CSF1R-mediated signaling activity including proliferation of leukemia lymphoblast cells.

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
CSF1; MCSF; CSF-1; M-CSF

Research Area :
Cancer

Pathway/Disease :
CSF Signaling Pathway

Species :
Human

CD Antigen :

References :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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