Name :
Human CSF1/MCSF Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CSF1 (colony stimulating factor 1), also known as MCSF (macrophage colony stimulating factor) is a single-pass type I transmembrane or secreted protein. The active form of CSF1 is a disulfide-linked homodimer, produced by proteolytic cleavage of membrane-bound precursors. As a cytokine, CSF1 controls the production, differentiation, and function of macrophages. CSF1 primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. CSF1 induces the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. CSF1 is required for male and female fertility. CSF1 induces reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Aberrant expression of CSF1 or its receptor CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in many types of cancers including breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R is implicated in inflammatory conditions, such as rheumatoid arthritis (RA), glomerulonephritis, atherosclerosis, and allograft rejection.
Gene Symbol :
CSF1; MCSF; CSF-1; M-CSF
NCBI Gene ID :
1435
Uniprot Entry :
P09603
Construct Details :
The recombinant human CSF1-Fc is expressed as a 388-amino acid protein consisting of Glu33 – Gln181 region of CSF1 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CSF1-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI 70 80 90 100 110 120 MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV 130 140 150 160 170 180 FNETKNLLDK DWNIFSKNCN NSFAECSSQG STTENLYFQG STGTHTCPPC PAPELLGGPS 190 200 210 220 230 240 VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST 250 260 270 280 290 300 YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT 310 320 330 340 350 360 KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ 370 380 GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 44.2 Estimated by SDS-PAGE under reducing condition (kDa): 50-55.
Calculated PI :
5.66
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 49110
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds its receptor human CSF1R (SKU#FCL2648) and induces CSF1R-mediated signaling activity including proliferation of leukemia lymphoblast cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CSF1; MCSF; CSF-1; M-CSF
Research Area :
Cancer
Pathway/Disease :
CSF Signaling Pathway
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Caspase-7/CASP7 Protein
CHRNA1 Protein
Popular categories:
Ubiquitin-Specific Peptidase 30
B Cell CD Proteins
