Name :
Human CD96/TACTILE Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD96, also known as Tactile (T cell-activated increased late expression), is a single pass type I transmembrane protein of the immunoglobulin superfamily (IgSF). The extracellular domain (ECD) of CD96 contains 2 V-type and 1 C-type Ig-like domains, which are highly glycosylated. An 80-kDa soluble form of CD96 is elevated in human serum during chronic hepatitis B. CD96 is expressed on activated T, NK and a subpopulation of B cells as well as on acute myeloid leukemia and myelodysplastic stem cells. CD96 is allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR) or CD155, a nectins and nectin-like protein family member serve to mediate cell-cell adhesion and cell migration. CD96 may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells. The most N-terminal Ig-like domain of CD96 binds to PVR. On NK cells, co-stimulatory molecules CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various members of nectin family. CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. CD96 mutations may cause a form of the C syndrome, a set of developmental anomalies in cranial bone, skin and viscera.
Gene Symbol :
NCBI Gene ID :
10225
Uniprot Entry :
P40200
Construct Details :
The recombinant human CD96-Fc fusion is expressed as a 719-amino acid protein consisting of Val22 – Gly502 region of CD96 (Uniprot#P40200-2) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD96-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 VWEKTVNTEE NVYATLGSDV NLTCQTQTVG FFVQMQWSKV TNKIDLIAVY HPQYGFYCAY 70 80 90 100 110 120 GRPCESLVTF TETPENGSKW TLHLRNMSCS VSGRYECMLV LYPEGIQTKI YNLLIQTHVT 130 140 150 160 170 180 ADEWNSNHTI EIEINQTLEI PCFQNSSSKI SSEFTYAWSV EDNGTQETLI SQNHLISNST 190 200 210 220 230 240 LLKDRVKLGT DYRLHLSPVQ IFDDGRKFSC HIRVGPNKIL RSSTTVKVFA KPEIPVIVEN 250 260 270 280 290 300 NSTDVLVERR FTCLLKNVFP KANITWFIDG SFLHDEKEGI YITNEERKGK DGFLELKSVL 310 320 330 340 350 360 TRVHSNKPAQ SDNLTIWCMA LSPVPGNKVW NISSEKITFL LGSEISSTDP PLSVTESTLD 370 380 390 400 410 420 TQPSPASSVS PARYPATSSV TLVDVSALRP NTTPQPSNSS MTTRGFNYPW TSSGTDTKKS 430 440 450 460 470 480 VSRIPSETYS SSPSGAGSTL HDNVFTSTAR AFSEVPTTAN GSTKTNHVHI TGIVVNKPKD 490 500 510 520 530 540 GSTTENLYFQ GSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH 550 560 570 580 590 600 EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 610 620 630 640 650 660 PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE 670 680 690 700 710 NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 80.1 Estimated by SDS-PAGE under reducing condition (kDa): 100-110.
Calculated PI :
6.47
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 108135
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds PVR/CD155/NECL5 (SKU#FCL1077) and neutralizes its mediated signaling activity.
Molecule Class :
1-pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD96; TACTILE
Research Area :
Immunology
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD96
References :
1. J. Immunol. 148:2600 (1992) 2. J. Immunol. 172:3994 (2004) 3. Proc. Natl. Acad. Sci. 104:11008 (2007) 4. Am. J. Hum. Genet. 81:835 (2007) 5. J. Biol. Chem. 284:2235 (2009)
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
RANKL/TNFSF11 Protein
TGF beta 1/TGFB1 Protein
Popular categories:
Cadherin-10
KLRB1F/CD161f
