Name :
Human 4-1BBL/CD137L/TNFSF9 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
4-1BBL (4-1BB ligand), also known as CD137L or TNFSF9, is single pass type II transmembrane protein that belongs to the TNF superfamily (TNFSF). 4-1BBL is a a bidirectional signal transducer that acts as a high affinity ligand for 4-1BB/CD137/TNFRSF9, a member of the TNF-receptor superfamily (TNFRSF). Members of the TNFRSF have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. The extracellular domain (ECD) of 4-1BBL has a jellyroll, β-sandwich tertiary structure that is similar to other TNFSF members. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. A soluble active form of 4-1BBL is detected in humans, presumably generated by MMP activity. 4-1BBL may signal through both 4-1BB and itself as its cytoplasmic tail participates in reverse signaling to induce apoptosis in T cells and secretion of cytokines such as IL6 and TNFα by monocytes. 4-1BBL/4-1BB signaling is involved in the antigen presentation process and in the generation of cytotoxic T cells. 4-1BBL/4-1BB interaction also provides a co-stimulatory signal to T cells, and increases T cell proliferation and cytokines production. 4-1BBL may play a key role in T cell recall response and maintaining T cell numbers. 4-1BBL is required for the optimal CD8 responses in CD8 T cells. 4-1BBL is expressed on cancer cells, and is thought to be involved in T cell-tumor cell interaction. 4-1BBL may also play a role in interactions between T-cells and B-cells/macrophages. 4-1BBL is implicated in cancers, infectious diseases and autoimmune diseases.
Gene Symbol :
4-1BBL; TNFSF9; CD137L; TNLG5A; 4-1BB-L
NCBI Gene ID :
8744
Uniprot Entry :
P41273
Construct Details :
The recombinant human 4-1BBL-Fc is expressed as a 412-amino acid protein consisting of Arg71 – Glu254 region of 4-1BBL with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human 4-1BBL-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT 70 80 90 100 110 120 KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS 130 140 150 160 170 180 EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS 190 200 210 220 230 240 PRSESTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF 250 260 270 280 290 300 NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT 310 320 330 340 350 360 ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP 370 380 390 400 410 PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
M.W. :
Calculated molecular mass (kDa): 45; Estimated by SDS-PAGE under reducing condition (kDa): ~45-50 with 2 distinct high MW bands around 75 kDa and 95 kDa.
Calculated PI :
6.68
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 52745
Endotoxin Level :
>85% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its receptor 4-1BB (SKU#FCL0100) with high affinity and induces its signaling activity.
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
4-1BBL; TNFSF9; CD137L; TNLG5A; 4-1BB-L
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD137L
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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