Name :
Human DcR3, Fc-fusion, Biotinylated, Recombinant
Description :
DcR3 (death decoy receptor), also known as decoy receptor for Fas ligand, TR6 and M68, is a member of the TNF receptor superfamily (TNFRSF6B). Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein that contains 4 TNFR cysteine-rich repeats. Expression of DcR3 has been reported in a wide array of normal tissues as well as primary tumors and tumor cell lines. DcR3 is detected in fetal lung, brain and liver and in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. DcR3 is highly expressed in primary tumors from colon, stomach, rectum, and esophagus as well as in SW480 colon cancer cell line. DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and TNF- like molecule 1A (TL1A). The principal function of DcR3 is the inhibition of FasL and LIGHT-induced apoptosis. In addition to DcR1, DcR2 and OPG, DcR3 is another TNFRSF member that modulates cytotoxic ligands-induced apoptosis. Overexpression of DcR3 may be a mechanism by which certain tumors escape immunecytotoxic attack. DcR3 may also modulate CNS- autoimmunity by interfering with Th17 responses via the blockade of Fas-FasL interaction.
Gene Symbol :
TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1; UNQ186/PRO212
NCBI Gene ID :
8771
Uniprot Entry :
Q95407
Construct Details :
The recombinant human DcR3-Fc fusion is expressed as a 499 amino acid protein consisting of Val30 – His300 region of DcR3 (UniProt accession #O95407) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human DcR3-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 VAETPTYPWR DAETGERLVC AQCPPGTFVQ RPCRRDSPTT CGPCPPRHYT QFWNYLERCR 70 80 90 100 110 120 YCNVLCGERE EEARACHATH NRACRCRTGF FAHAGFCLEH ASCPPGAGVI APGTPSQNTQ 130 140 150 160 170 180 CQPCPPGTFS ASSSSSEQCQ PHRNCTALGL ALNVPGSSSH DTLCTSCTGF PLSTRVPGAE 190 200 210 220 230 240 ECERAVIDFV AFQDISIKRL QRLLQALEAP EGWGPTPRAG RAALQLKLRR RLTELLGAQD 250 260 270 280 290 300 GALLVRLLQA LRVARMPGLE RSVRERFLPV HSTGTHTCPP CPAPELLGGP SVFLFPPKPK 310 320 330 340 350 360 DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV 370 380 390 400 410 420 LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL 430 440 450 460 470 480 VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 490 HEALHNHYTQ KSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 53.3; Estimated by SDS-PAGE under reducing condition (kDa): ~60
Calculated PI :
8.41
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 59495
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to human FasL and Light. Inhibits FasL-induced apoptosis in human Jurkat acute T cell leukemia cells.
Molecule Class :
Decoy Receptor (secreted)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
DcR3; TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1; UNQ186/PRO212
Research Area :
Immunology
Pathway/Disease :
Apoptosis
Species :
Human
CD Antigen :
References :
1. Nature 396:699 (1998). 2. J. Biochem. Chem. 274:13733 (1999). 3. Proc. Natl. Acad. Sci. 97:1230 (2000). 4. J Am Soc Nephrol. 19: 1025 (2008). 5. J Neuroimmunol. 209: 57 (2009).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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