Name :
Human DR4 ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
DR4 (death receptor 4), also known as CD261, TRAILR1 and APO-2, is a single pass type 1 transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF10A). DR4 contains an extracellular region with 3 cysteine-rich repeats and a cytoplasmic death domain (DD). DR4 is widely expressed with high levels in spleen, peripheral blood leukocytes, small intestine and thymus. DR4 is detected mainly in damaged, infected and malignant cells. DR4 is also expressed on hemopoietic cells including activated T-cells and influences activities such as monocyte migration. DR4 is one of 5 receptors for TRAIL (tumor necrosis factor-related apoptosis inducing ligand), also known as Apo2L (Apo-2 ligand) and TNFSF10. TRAIL preferentially induces apoptosis in tumor cell lines, but not in normal cells. TRAIL binds at least 5 different receptors with characteristic TNFR cysteine-rich repeats. DR4/TRAILR1 is most closely related to DR5/TRAILR2 sharing 59% amino acid identity within the cytoplasmic DD. DR4 transduces cell death signal and induces apoptosis by recruiting a cytosolic deathinducing signaling complex (DISC) that includes the adaptor FADD and caspase 8 and caspase 10. DR4 is thought to function in immune surveillance, inducing apoptosis in cancer cells but not normal cells. DR4 surface expression correlates more closely than that of DR5 with TRAIL induction of apoptosis in tumor cells.
Gene Symbol :
TNFRSF10A; APO2; CD261; TRAILR1; TRAIL-R1; TRAILR-1
NCBI Gene ID :
8797
Uniprot Entry :
O00220
Construct Details :
The recombinant human DR4-Fc fusion is expressed as a 445 amino acid protein consisting of Ala24 – Asn239 region of DR4 (UniProt accession #O00220) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human DR4-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ASGTEAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS 70 80 90 100 110 120 PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSEHPG 130 140 150 160 170 180 ACNRCTEGVG YTNASNNLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM 190 200 210 220 230 240 CRKCSRGCPR GMVKVKDCTP WSDIECVHKE SGNGHNGSTG THTCPPCPAP ELLGGPSVFL 250 260 270 280 290 300 FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV 310 320 330 340 350 360 VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ 370 380 390 400 410 420 VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV 430 440 FSCSVMHEAL HNHYTQKSLS LSPGK
M.W. :
Calculated molecular mass (kDa): 48.5; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
8.74
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 54650
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand human TRAIL and inhibits TRAIL-mediated signaling activity including TRAIL-induced cytotoxicity in L-929 mouse fibroblast cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
DR4; TNFRSF10A; APO2; CD261; TRAILR1; TRAIL-R1; TRAILR-1
Research Area :
Immunology
Pathway/Disease :
Apoptosis
Species :
Human
CD Antigen :
CD261
References :
1. Science 276:111 (1997). 2. J. Immunol. 175:1232 (2005). 3. Mol. Cancer Ther. 6:2591 (2007). 4. Clin. Cancer Res. 15:5584 (2009). 5. Oncogene 29:4848 (2010).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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