Name :
Human EDAR Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
EDAR (ectodysplasin A receptor), also known as Anhidrotic ectodysplasin receptor 1, Ectodysplasin-A receptor, Ectodermal dysplasia receptor, Downless homolog, and DL, is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF). The extracellular domain (ECD) of EDAR contains three TNFR cysteinerich repeats and the cytoplasmic domain contains a region with homology to the death domain (DD) found in other TNFRSF members. EDAR is detected in fetal kidney, lung, skin and cultured neonatal epidermal keratinocytes but not detected in lymphoblast and fibroblast cells. Within the TNFRSF, EDAR shares the highest homologies with XEDAR/EDA2R (TNFRSF27) and TROY (TNFRSF19). EDAR is a receptor for the soluble ligand ectodysplasin A isoform A1 (EDA-A1) but not for isoform A2 (EDA-A2), which binds to EDA2R/XEDAR, an Xlinked TNFRSF member with high homology to EDAR. Other than a 2 amino acid deletion in its TNFlike domain, EDAA2 is identical to EDAA1. EDAR can activate the NF-kB, JNK, and caspase-independent cell death pathways. EDAR is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia (HED), which is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. Defects in EDAR are also the cause of ectodermal dysplasia type 3 (ED3) which is an autosomal dominant condition characterized by hypotrichosis, abnormal or missing teeth, and hypohidrosis due to the absence of sweat glands.
Gene Symbol :
DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
NCBI Gene ID :
10913
Uniprot Entry :
Q9UNE0
Construct Details :
The recombinant human EDAR-Fc fusion is expressed as a 389 amino acid protein consisting of Glu27 – Ala187 region of EDAR (UniProt accession #Q9UNE0) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human EDAR-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EYSNCGENEY YNQTTGLCQE CPPCGPGEEP YLSCGYGTKD EDYGCVPCPA EKFSKGGYQI 70 80 90 100 110 120 CRRHKDCEGF FRATVLTPGD MENDAECGPC LPGYYMLENR PRNIYGMVCY SCLLAPPNTK 130 140 150 160 170 180 ECVGATSGAS ANFPGTSGSS TLSPFQHAHK ELSGQGHLAT ASTGTHTCPP CPAPELLGGP 190 200 210 220 230 240 SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS 250 260 270 280 290 300 TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM 310 320 330 340 350 360 TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ 370 380 QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 42.9; Estimated by SDS-PAGE under reducing condition (kDa): 60-65
Calculated PI :
5.81
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 53050
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand EDAA1 and blocks EDA-A1-mediated signaling activity.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
EDAR; DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
Research Area :
Development
Pathway/Disease :
Epidermis Development
Species :
Human
CD Antigen :
References :
1. Nat. Genet. 22:366 (1999). 2. Nat. Genet. 22:370 (1999). 3. Proc. Natl. Acad. Sci. 94:13069 (1997). 4. Science 290:523 (2000). 5. J. Biol. Chem. 276:18819 (2001). 6. Hum. Mol. Genet. 17: 835 (2008).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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