Name :
Human LTBR Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
LTβR (Lymphotoxin beta receptor), also known as TNFR3 or TNF Rrp, is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF3). LTβR contains4 cysteine-rich repeats within the extracellular domain (ECD) characteristic of the TNFRSF. LTβR ligands include homotrimeric of LIGHT/TNFSF14 and heterotrimeric lymphotoxin-α1/β2. LTβR is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. LTβR is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures. Secondary immune tissues are absent in LTβR-deficient mice. Depending on the cell type, activation of LTβR has been shown to induce canonical or alternative NF-κB pathways. Soluble LTβR can be formed by proteolytic cleavage of the ECD, and act as an antagonist to inhibit autoimmunity. LTβR regulates cell growth and can initiate inflammation-related carcinogenesis. It is expressed in hepatocytes, is up-regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis.
Gene Symbol :
TNFRSF3; CD18; TNFCR; TNFR3; D12S370; TNFR-RP; TNFR2-RP; LT-BETA-R; TNF-R-III
NCBI Gene ID :
4055
Uniprot Entry :
P36941
Construct Details :
The recombinant human LTβR-Fc fusion is expressed as a 420 amino acid protein consisting of Gln31 – Thr234 region of LTβR (UniProt accession #P36941) and a C-terminal Fc from human IgG1, which exists as a dimer/tetramer/oligomer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human LTβR-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QAVPPYASEN QTCRDQEKEY YEPQHRICCS RCPPGTYVSA KCSRIRDTVC ATCAENSYNE 70 80 90 100 110 120 HWNYLTICQL CRPCDPVMGL EEIAPCTSKR KTQCRCQPGM FCAAWALECT HCELLSDCPP 130 140 150 160 170 180 GTEAELKDEV GKGNNHCVPC KAGHFQNTSS PSARCQPHTR CENQGLVEAA PGTAQSDTTC 190 200 210 220 230 240 KNPLEPLPPE MSGTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS 250 260 270 280 290 300 HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA 310 320 330 340 350 360 LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP 370 380 390 400 410 420 ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 46.8; Estimated by SDS-PAGE under reducing condition (kDa): 55-60
Calculated PI :
6.26
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 57100
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand LIGHT and lymphotoxin-α1/β2. Inhibits LIGHT/lymphotoxin-α1/β2-mediated biological activities including NF-κB activation and cytotoxicity.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TNFRSF3; CD18; TNFCR; TNFR3; D12S370; TNFR-RP; TNFR2-RP; LT-BETA-R; TNF-R-III
Research Area :
Immunology
Pathway/Disease :
Lymphoid Organogenesis
Species :
Human
CD Antigen :
CD18
References :
1. Science 264:707 (1994). 2. J. Immunol. 155:5280 (1995). 3. J. Exp. Med. 198:757 (2003). 4. Oncogene 29:5006 (2010). 5. Nat. Rev. Immunol. 10:664 (2010). 6. J. Immunol. 186:5047 (2011).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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