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Name :
Human M6PR Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Description :
The mannose 6-phophate receptors (MPRs) are single-pass type I transmembrane glycoproteins that target the newly synthesized hydrolases from the Golgi complex and the cell surface to lysosomes. There are two different MPRs, one of ~300kDa termed CI-MPR (cation-independent) and a smaller, dimeric receptor of ~46kDa termed CD-MPR (cation-depnendent). Both MPRss are P-type lectins that bind terminal mannose 6-phosphate (M6P) with similar affinity (CI-MPR = 7 μM, CD-MPR = 8 μM) and contain similar cytoplasmic signals for intracellular trafficking. CD-MPR shows greatly enhanced binding to M6P in the presence of divalent cations, such as manganese. The MPRs, when bound to their cargo via M6P, are recognized by the GGA family of clathrin adaptor proteins and accumulate in forming clathrin-coated vesicles. The vesicles are trafficked to the early endosome where, in the relatively low pH environment of the endosome, the MPRs release their cargo. The cargo proteins are then trafficked to the lysosome via the late endosome independently of the MPRs. CD-MPR is also known as M6PR that contain a 157 amino-acid M6P-binding-domain in the extracellular/luminal region. CI-MPR was also identified as the cell surface receptor for insulin-like growth factor 2 (IGF2R/CD222). IGF2R consists of 15 repeats homologous to the M6P-binding domain present in M6PR/CD-MPR and two of these 15 repeats are responsible for binding to M6P.

Gene Symbol :
M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; MPRD

NCBI Gene ID :
4074

Uniprot Entry :
P20645

Construct Details :
The recombinant human M6PR-Fc is expressed as a 387 amino acid protein consisting of Thr27 – Glu182 region of M6PR (UniProt #P20645) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

Source :
Human cells stably expressing human M6PR-Fc and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 TEEKTCDLVG EKGKESEKEL ALVKRLKPLF NKSFESTVGQ GSDTYIYIFR VCREAGNHTS 70 80 90 100 110 120 GAGLVQINKS NGKETVVGRL NETHIFNGSN WIMLIYKGGD EYDNHCGKEQ RRAVVMISCN 130 140 150 160 170 180 RHTLADNFNP VSEERGKVQD CFYLFEMDSS LACSPESTGS TGTHTCPPCP APELLGGPSV 190 200 210 220 230 240 FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY 250 260 270 280 290 300 RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK 310 320 330 340 350 360 NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG 370 380 NVFSCSVMHE ALHNHYTQKS LSLSPGK

M.W. :
Calculated molecular mass 43.3 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa probably due to glycosylation

Calculated PI :
6.42

Calculated Extinction Coefficients :

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; MPRD

Research Area :
Glycobiology

Pathway/Disease :
Lysosome Transporter

Species :
Human

CD Antigen :

References :

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