Name :
Human PD-1 protein, ECD (extracellular domain), biotinylated, recombinant
Description :
Programmed death-1 (PD-1), also known as programmed cell death 1 (PDCD1) or CD279, is a single pass type I transmembrane glycoprotein of the Ig superfamily. It is an immunoreceptor in the CD28/CTLA-4 family with an IgV-type extracellular domain showing 21–33% sequence identity with CTLA-4, CD28 and ICOS. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or inhibit T cell activation (CTLA4 and PD1). The cytoplasmic domain of PD-1 contains two tyrosine residues, a membrane-proximal immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). ITIM is widely found in immunoinhibitory receptors and the membrane-proximal tyrosine residue may play a central role for the inhibitory function of PD-1. PD-1 negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase upon engaging with either of two ligands, PD-L1 or PD-L2. It inhibits the T-cell proliferation and production of related cytokines such as IL-1, IL-4, IL-10 and IFN-γ. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a negative regulator of T- cell effector mechanisms, PD-1 may lead to suppression of anti-tumor immunity. Blockade of PD-1 inhibitory activity with antibodies enhances anti-tumor immunity in vivo. PD-1 has been proposed as a promising target for cancer immunotherapy.
Gene Symbol :
NCBI Gene ID :
5133
Uniprot Entry :
Q15116
Construct Details :
The recombinant human PD-1 ECD is expressed as a 159-amino acid protein consisting of Pro21 – Thr168 region of PD-1 (UniProt accession #Q15116) and a C-terminal His-tag. It contains 4 potential N-linked glycosylation sites.
Source :
Human cells stably expressing PD-1 ECD and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
PGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQD CRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAG QFQTSTGHHHHHHHH
M.W. :
Calculated molecular mass 17.9 kDa; estimated by SDS-PAGE under reducing condition 45-50 kDa probably due to glycosylation.
Calculated PI :
8.73
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand PD-L1 extracellular domain proteins (SKU#: FCL0781 and FCL784) and anti-PD-1 human IgG1 monoclonal antibody (SKU#: MAB1732) with high affinity KD < 1 nM (see the attached technical data for ELISA).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD279, PDCD1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD279
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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