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Name :
Human HER3 protein (extracellular domain), Fc-fusion, recombinant

Description :
HER3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases (RTK). Four members of the EGFR family have been identified: EGFR (ERBB1, HER1), HER2 (ERBB2), HER3 (ERBB3) and HER4 (ERBB4). They typically contain an extracellular ligand binding domain (ECD), a transmembrane domain (TM), and an intracellular kinase domain that can interact with a multitude of signaling molecules and exhibit both ligand-dependent and ligand-independent activity. EGFR signaling is initiated by ligand binding to the extracellular ligand binding domain. This initiates receptor homo-/hetero-dimerization and autophosphorylation by the intracellular kinase domain, resulting in receptor activation. Following activation, phosphorylation of cytoplasmic substrates occurs and a signaling cascade is initiated that drives many cellular responses, including changes in gene expression, cytoskeletal rearrangement, anti-apoptosis and increased cell proliferation. HER3 binds neuregulins NRG1 and NRG2, but lacks an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, heterodimerization with other EGF receptor family members, which have kinase activity, leads to the activation of pathways. Amplification or overexpression of this gene has been reported in several cancers, including lung, prostate, bladder, and breast tumors. HER3 is also genetically linked to type 1 diabetes and lethal congenital contracture syndrome-2 (LCCS2).

Gene Symbol :

NCBI Gene ID :
2065 (human)

Uniprot Entry :
P21860 (human)

Construct Details :
Expressed as a 860 amino acid protein consisting of Ser20-Thr643 region of HER3 and a C-terminal Fc Fusion from human IgG1, which exists as a dimmer under non-reducing condition (see the gel image above, labeled as DTT “-“)

Source :
Human cells stably expressing HER3-Fc and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :

M.W. :
Calculated molecular mass (kDa): 95.1; Estimated by SDS-PAGE under reducing condition (kDa): 98

Calculated PI :

Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 102740

Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)

Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
It was reported to inhibit the biological activity of Neuregulin-1 on MCF‑7 human breast cancer cells

Molecule Class :
Receptor Tyrosine Kinase (RTK)

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
HER3; ERBB3; LCCS2; ErbB-3; c-erbB3; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3

Research Area :

Pathway/Disease :
EGF/PDGF Signaling Pathway

Species :

CD Antigen :

References :
1. J. Biol. Chem. 269: 14303-14306, 1994.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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