Name :
Human RANK Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
RANK (receptor activator of NFκB), also known as CD265 and ODFR, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF11A). RANK contains 4 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). RANK is ubiquitously expressed with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. TRANCE (TNFrelated activationinduced cytokines), also known as OPGL (osteoprotegerin ligand) and ODF (osteoclast differentiation factor), is the ligand for RANK (RANKL). RANK and RANKL are important regulators of the interaction between T cells and dendritic cells for the enhancement of T cell growth and dendritic cell function. They are also essential mediators for osteoclast and lymph node development. Mutations in RANK are associated with familial expansile osteolysis (FEO), Paget disease of bone type 2 (PDB2), and osteopetrosis autosomal recessive type 7 (OPTB7). FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. OPTB7 is characterized by abnormally dense bone, due to defective resorption of immature bone. Soluble RANK is able to block RANKL-induced biological activity. RANK can interact with various TRAF family proteins, through which this receptor induces the activation of NF-κB and JNK pathways.
Gene Symbol :
TNFRSF11A; CD265; FEO; OFE; ODFR; OSTS; PDB2; OPTB7; TRANCER; LOH18CR1
NCBI Gene ID :
8792
Uniprot Entry :
Q9Y6Q6
Construct Details :
The recombinant human RANK-Fc fusion is expressed as a 411 amino acid protein consisting of Ile30 – Pro212 region of RANK (UniProt accession #Q9Y6Q6) and a C-terminal Fc from human IgG1, which exists as a dimer/tetramer/oligomer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human RANK-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 IAPPCTSEKH YEHLGRCCNK CEPGKYMSSK CTTTSDSVCL PCGPDEYLDS WNEEDKCLLH 70 80 90 100 110 120 KVCDTGKALV AVVAGNSTTP RRCACTAGYH WSQDCECCRR NTECAPGLGA QHPLQLNKDT 130 140 150 160 170 180 VCKPCLAGYF SDAFSSTDKC RPWTNCTFLG KRVEHHGTEK SDAVCSSSLP ARKPPNEPHV 190 200 210 220 230 240 YLPSTGTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN 250 260 270 280 290 300 WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI 310 320 330 340 350 360 SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP 370 380 390 400 410 VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 45.7; Estimated by SDS-PAGE under reducing condition (kDa): ~55
Calculated PI :
7.23
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 62475
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand human RANKL and inhibits RANKL-induced signaling activity including osteoclast differentiation in mouse monocyte/macrophage cells RAW 264.7.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TNFRSF11A; CD265; FEO; OFE; ODFR; OSTS; PDB2; OPTB7; TRANCER; LOH18CR1
Research Area :
Immunology
Pathway/Disease :
Osteoclastogenesis
Species :
Human
CD Antigen :
CD265
References :
1. Nature 390:175 (1997). 2. J. Biol. Chem. 273: 20551 (1998). 3. J. Biol. Chem. 273: 34120 (1998). 4. Nat. Genet. 24:45 (2000). 5. Am. J. Hum. Genet. 83:64 (2008).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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